Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues

M Sammar; P J Babin; M Durliat; I Meiri; I Zchori; Abigail Elizur; E Lubzens

doi:10.1006/gcen.2001.7636

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Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues

Journal article

Peer reviewed

Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues

M Sammar, P J Babin, M Durliat, I Meiri, I Zchori, Abigail Elizur and E Lubzens

General and Comparative Endocrinology, Vol.123(1), pp.51-61

2001

DOI: https://doi.org/10.1006/gcen.2001.7636

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Abstract

Clinical Sciences

retinol-binding protein

cDNA

trout

vitellogenin

liver

17β-estradiol

Retinoids are important regulatory signaling molecules during embryonic development. The molecular properties of rainbow trout (Oncorhynchus mykiss) retinol-binding protein (rtRBP), the specific retinol carrier in vertebrate plasma, were studied to elucidate its role in transporting retinols to developing fish oocytes. A 954-nucleotide rtRBP cDNA was cloned from the liver coding for a 176-amino-acid (aa) mature protein, with an estimated molecular mass of 20,267 Da. The nucleotide sequence suggests a putative 16-aa signal peptide and shows all the aa residues that were previously identified as critical for the retinol binding pocket. Five of the eight amino acid residues that are associated with the interaction of RBP and transthyretin in mammalian and nonmammalian species are conserved. The deduced aa sequence of rtRBP shows 60-66% identity with zebrafish, chicken, mouse, rat, horse, bovine, and human RBPs and 56% identity with Xenopus RBP. Northern blot analysis revealed a 1.1-kb hepatic mRNA transcript. RBP is highly expressed in the liver, but low levels were also detected in the spleen, kidney, ovary, and brain. In the rainbow trout, 17β-estradiol treatment led to a decrease in the RBP mRNA signal relative to that of the controls. The efficacy of the 17β-estradiol treatment was verified by an induction of vitellogenin (VTG) mRNA expression in the liver and occurrence of VTG in the plasma.

Details

Title: Retinol binding protein in rainbow trout: Molecular properties and mRNA expression in tissues
Authors: M Sammar (Author) - Israel Oceanographic and Limnological Research
P J Babin (Author) - Universite Bordeaux
M Durliat (Author) - Universite Paris-Sud
I Meiri (Author) - Israel Oceanographic and Limnological Research
I Zchori (Author) - Tel-Aviv University
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
E Lubzens (Author) - Israel Oceanographic and Limnological Research
Publication details: General and Comparative Endocrinology, Vol.123(1), pp.51-61
Publisher: Academic Press
Date published: 2001
DOI: 10.1006/gcen.2001.7636
ISSN: 0016-6480
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449462202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism; Zoology

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