Journal article
Repeated reuse of deoxyribozyme-based logic gates
Nano Letters, Vol.19(11), pp.7655-7661
2019
PMID: 31615207
Abstract
Deoxyribozymes (DNAzymes) have demonstrated significant capacity for biocomputing, and hold promise for information processing within advanced biological devices if several key capabilities are developed. One required capability is reuse; having DNAzyme logic gates be cyclically, and controllably, activated and deactivated. We designed an oligonucleotide-based system for DNAzyme reuse that could (1) remove previously bound inputs by addition of complementary oligonucleotides via toe-hold mediated binding, and (2) diminish output signal through the addition of quencher-labelled oligonucleotides complementary to the fluorophore-bound substrate. Our system demonstrated, for the first time, the ability for DNAzymes to have their activity toggled, with activity returning to 90-125% of original activity. This toggling could be performed multiple times with control being exerted over when the toggling occurs, with three clear cycles observed before the variability in activity became too great. Our data also demonstrated that fluorescent output of the DNAzymes activity could be actively removed and regenerated. This reuse system can increase the efficiency of DNAzyme-based logic circuits by reducing the number of redundant oligonucleotides, and is critical for future development of reusable bio-devices controlled by logical operations.
Details
- Title
- Repeated reuse of deoxyribozyme-based logic gates
- Authors
- Bradley Harding (Author) - University of the Sunshine CoastNina M Pollak (Author) - University of the Sunshine CoastDarko Stefanovic (Author) - University of New Mexico, United StatesJoanne Macdonald (Author) - University of the Sunshine Coast
- Publication details
- Nano Letters, Vol.19(11), pp.7655-7661
- Publisher
- American Chemical Society
- Date published
- 2019
- DOI
- 10.1021/acs.nanolett.9b02326
- ISSN
- 1530-6984
- PMID
- 31615207
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451507602621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Chemistry, Multidisciplinary
- Chemistry, Physical
- Materials Science, Multidisciplinary
- Nanoscience & Nanotechnology
- Physics, Applied
- Physics, Condensed Matter
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