Removal of Fecal Indicators, Pathogenic Bacteria, Adenovirus, Cryptosporidium and Giardia (oo)cysts in Waste Stabilization Ponds in Northern and Eastern Australia

Maxim Sheludchenko; Anna Padovan; Mohammad Katouli; Helen Stratton

doi:10.3390/ijerph13010096

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Removal of Fecal Indicators, Pathogenic Bacteria, Adenovirus, Cryptosporidium and Giardia (oo)cysts in Waste Stabilization Ponds in Northern and Eastern Australia

Journal article

Open access

Peer reviewed

Removal of Fecal Indicators, Pathogenic Bacteria, Adenovirus, Cryptosporidium and Giardia (oo)cysts in Waste Stabilization Ponds in Northern and Eastern Australia

Maxim Sheludchenko, Anna Padovan, Mohammad Katouli and Helen Stratton

International Journal of Environmental Research and Public Health, Vol.13(1), 96

2016

DOI: https://doi.org/10.3390/ijerph13010096

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Abstract

waste stabilization pond (WSP)

maturation pond

E. coli

enterococci

Campylobacter jejuni

Salmonella enterica

Giardia cysts

Maturation ponds are used in rural and regional areas in Australia to remove the microbial loads of sewage wastewater, however, they have not been studied intensively until present. Using a combination of culture-based methods and quantitative real-time PCR, we assessed microbial removal rates in maturation ponds at four waste stabilization ponds (WSP) with (n = 1) and without (n = 3) baffles in rural and remote communities in Australia. Concentrations of total coliforms, E. coli, enterococci, Campylobacter spp., Salmonella spp., F+ RNA coliphage, adenovirus, Cryptosporidium spp. and Giardia (oo) cysts in maturation ponds were measured at the inlet and outlet. Only the baffled pond demonstrated a significant removal of most of the pathogens tested and therefore was subjected to further study by analyzing E. coli and enterococci concentrations at six points along the baffles over five sampling rounds. Using culture-based methods, we found a decrease in the number of E. coli and enterococci from the initial values of 100,000 CFU per 100 mL in the inlet samples to approximately 1000 CFU per 100 mL in the outlet samples for both bacterial groups. Giardia cysts removal was relatively higher than fecal indicators reduction possibly due to sedimentation.

Details

Title: Removal of Fecal Indicators, Pathogenic Bacteria, Adenovirus, Cryptosporidium and Giardia (oo)cysts in Waste Stabilization Ponds in Northern and Eastern Australia
Authors: Maxim Sheludchenko (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Anna Padovan (Author) - Charles Darwin University
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Helen Stratton (Author) - Griffith University
Publication details: International Journal of Environmental Research and Public Health, Vol.13(1), 96; 18
Publisher: MDPI AG
Date published: 2016
DOI: 10.3390/ijerph13010096
ISSN: 1660-4601
Copyright note: Copyright © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449252102621
Output Type: Journal article

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