Journal article
Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins
Fish Physiology and Biochemistry, Vol.49, pp.911-923
2023
Abstract
The two gonadotropins, FSH and LH, stimulate growth and development of the gonads through gonadal biosynthesis of steroid hormones and growth factors. To date, cDNA sequences encoding gonadotropin subunits have been isolated and characterized from a large number of fish species. Recently, we successfully cloned and characterized gonadotropins (LHβ, FSHβ, and GPα) from the pituitary glands of the catfish, Heteropneustes fossilis. In the present study, we describe herein the production of recombinant stinging catfish, H. fossilis (hf) FSH (rhfFSH) and LH (rhfLH) using the methylotrophic yeast P. pastoris expression system. We further explored the hypothesis that the recombinant gonadotropins can modulate the hypothalamus-pituitary-ovarian (HPO) axis genes (avt, it, gnrh2, kiss2, and cyp19a1a) and regulate their transcriptional profile and steroid levels in relation to their annual developmental stage during preparatory and pre-spawning phases under in-vitro conditions. We found that the different concentrations of recombinant rhfFSH and rhfLH significantly stimulated E2 levels in the preparatory and prespawning season, and also upregulated gonadal aromatase gene expression in a dose dependent manner. Our results demonstrate that the yeast expression system produced biologically active recombinant catfish gonadotropins, enabling the study of their function in the catfish.
Details
- Title
- Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins
- Authors
- Joseph Aizen (Corresponding Author) - Ruppin Academic CenterSandhya Sharma (Author) - Banaras Hindu UniversityAbigail Elizur (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationK P Joy (Author) - Cochin University of Science and TechnologyRadha Chaube (Author) - Banaras Hindu University
- Publication details
- Fish Physiology and Biochemistry, Vol.49, pp.911-923
- Publisher
- Springer Dordrecht
- DOI
- 10.1007/s10695-023-01230-4
- ISSN
- 1573-5168
- Organisation Unit
- Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99743698802621
- Output Type
- Journal article
Metrics
8 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Fisheries
- Physiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites