Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins

Joseph Aizen; Sandhya Sharma; Abigail Elizur; K P Joy; Radha Chaube

doi:10.1007/s10695-023-01230-4

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Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins

Journal article

Peer reviewed

Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins

Joseph Aizen, Sandhya Sharma, Abigail Elizur, K P Joy and Radha Chaube

Fish Physiology and Biochemistry, Vol.49, pp.911-923

2023

DOI: https://doi.org/10.1007/s10695-023-01230-4

PMID: 37548828

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Abstract

Catfish

Recombinant LH and FSH

In-vitro culture

Yeast expression system

Steroid hormones

The two gonadotropins, FSH and LH, stimulate growth and development of the gonads through gonadal biosynthesis of steroid hormones and growth factors. To date, cDNA sequences encoding gonadotropin subunits have been isolated and characterized from a large number of fish species. Recently, we successfully cloned and characterized gonadotropins (LHβ, FSHβ, and GPα) from the pituitary glands of the catfish, Heteropneustes fossilis. In the present study, we describe herein the production of recombinant stinging catfish, H. fossilis (hf) FSH (rhfFSH) and LH (rhfLH) using the methylotrophic yeast P. pastoris expression system. We further explored the hypothesis that the recombinant gonadotropins can modulate the hypothalamus-pituitary-ovarian (HPO) axis genes (avt, it, gnrh2, kiss2, and cyp19a1a) and regulate their transcriptional profile and steroid levels in relation to their annual developmental stage during preparatory and pre-spawning phases under in-vitro conditions. We found that the different concentrations of recombinant rhfFSH and rhfLH significantly stimulated E2 levels in the preparatory and prespawning season, and also upregulated gonadal aromatase gene expression in a dose dependent manner. Our results demonstrate that the yeast expression system produced biologically active recombinant catfish gonadotropins, enabling the study of their function in the catfish.

Details

Title: Regulation of steroid production and key genes in catfish Heteropneustes fossilis using recombinant gonadotropins
Authors: Joseph Aizen (Corresponding Author) - Ruppin Academic Center
Sandhya Sharma (Author) - Banaras Hindu University
Abigail Elizur (Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
K P Joy (Author) - Cochin University of Science and Technology
Radha Chaube (Author) - Banaras Hindu University
Publication details: Fish Physiology and Biochemistry, Vol.49, pp.911-923
Publisher: Springer Dordrecht
Date published: 2023
DOI: 10.1007/s10695-023-01230-4
ISSN: 1573-5168
PMID: 37548828
Organisation Unit: Centre for Bioinnovation
Language: English
Record Identifier: 99743698802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Fisheries; Physiology

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