Regulation of gonadotropin-releasing hormone (GnRH)-receptor gene expression in tilapia: Effect of GnRH and dopamine

B Levavi-Sivan; H Safarian; H Rosenfeld; Abigail Elizur; A Avitan

doi:10.1095/biolreprod.103.021998

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Regulation of gonadotropin-releasing hormone (GnRH)-receptor gene expression in tilapia: Effect of GnRH and dopamine

Journal article

Peer reviewed

Regulation of gonadotropin-releasing hormone (GnRH)-receptor gene expression in tilapia: Effect of GnRH and dopamine

B Levavi-Sivan, H Safarian, H Rosenfeld, Abigail Elizur and A Avitan

Biology of Reproduction, Vol.70(6), pp.1545-1551

2004

DOI: https://doi.org/10.1095/biolreprod.103.021998

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Abstract

Medical Physiology

dopamine

gonadotropin-releasing hormone receptor

gonadotropin-releasing hormone

pituitary

The present work was designed to study certain aspects of the endocrine regulation of gonadotropin-releasing hormone receptor (GnRH-R) in the pituitary of the teleost fish tilapia. A GnRH-R was cloned from the pituitary of hybrid tilapia (taGnRH-R) and was identified as a typical seven-transmembrane receptor. Northern blot analysis revealed a single GnRH-R transcript in the pituitary of approximately 2.3 kilobases. The taGnRH-R mRNA levels were significantly higher in females than in males. Injection of the salmon GnRH analog (sGnRHa; 5–50 µg/kg) increased the steady-state levels of taGnRH-R mRNA, with the highest response recorded at 25 µg/kg and at 36 h. At the higher dose of sGnRHa (50 µg/kg), taGnRH-R transcript appeared to be down-regulated. Exposure of tilapia pituitary cells in culture to graded doses (0.1–100 nM) of seabream (sbGnRH = GnRH I), chicken II (cGnRH II), or salmon GnRH (sGnRH = GnRH III) resulted in a significant increase in taGnRH-R mRNA levels. The highest levels of both LH release and taGnRH-R mRNA levels were recorded after exposure to cGnRH II and the lowest after exposure to sbGnRH. The dopamine-agonist quinpirole suppressed LH release and mRNA levels of taGnRH-R, indicating an inhibitory effect on GnRH-R synthesis. Collectively, these data provide evidence that GnRH in tilapia can up- regulate, whereas dopamine down-regulates, taGnRH-R mRNA levels.

Details

Title: Regulation of gonadotropin-releasing hormone (GnRH)-receptor gene expression in tilapia: Effect of GnRH and dopamine
Authors: B Levavi-Sivan (Author) - Hebrew University of Jerusalem, Israel
H Safarian (Author) - Hebrew University of Jerusalem, Israel
H Rosenfeld (Author) - Israel Oceanographic and Limnological Research, Israel
Abigail Elizur (Author) - Queensland Department of Primary Industries
A Avitan (Author) - Hebrew University of Jerusalem, Israel
Publication details: Biology of Reproduction, Vol.70(6), pp.1545-1551
Publisher: Society for the Study of Reproduction
Date published: 2004
DOI: 10.1095/biolreprod.103.021998
ISSN: 0006-3363
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450021702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Reproductive Biology

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