Files and links (2)
Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem-stromal cells is restored by aldehyde dehydrogenase agonists3.95 MB
Published VersionCC BY V4.0, Open Access
Journal article
Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem/stromal cells is restored by aldehyde dehydrogenase agonists
Scientific Reports, Vol.7, pp.1-15
2017
PMCID: PMC5304324
PMID: 28205523
Abstract
High resistance to oxidative stress is a common feature of mesenchymal stem/stromal cells (MSC) and is associated with higher cell survival and ability to respond to oxidative damage. Aldehyde dehydrogenase (ALDH) activity is a candidate "universal" marker for stem cells. ALDH expression was significantly lower in decidual MSC (DMSC) isolated from preeclamptic (PE) patients. ALDH gene knockdown by siRNA transfection was performed to create a cell culture model of the reduced ALDH expression detected in PE-DMSC. We showed that ALDH activity in DMSC is associated with resistance to hydrogen peroxide (H2O2)-induced toxicity. Our data provide evidence that ALDH expression in DMSC is required for cellular resistance to oxidative stress. Furthermore, candidate ALDH activators were screened and two of the compounds were effective in upregulating ALDH expression. This study provides a proof-of-principle that the restoration of ALDH activity in diseased MSC is a rational basis for a therapeutic strategy to improve MSC resistance to cytotoxic damage.
Details
- Title
- Reduced aldehyde dehydrogenase expression in preeclamptic decidual mesenchymal stem/stromal cells is restored by aldehyde dehydrogenase agonists
- Authors
- Gina D. Kusuma (Author) - The University of MelbourneMohamed H. Abumaree (Author) - King Saud bin Abdulaziz University for Health SciencesAnthony V. Perkins (Author) - Griffith UniversityShaun P. Brennecke (Author) - The University of MelbourneBill Kalionis (Corresponding Author) - The University of Melbourne
- Publication details
- Scientific Reports, Vol.7, pp.1-15
- Publisher
- Nature Publishing group
- Date published
- 2017
- DOI
- 10.1038/srep42397
- ISSN
- 2045-2322
- PMID
- 28205523; PMC5304324
- Copyright note
- This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Grant note
- Royal Women's Hospital Foundation Australian Stem Cell Centre postgraduate scholarship RC08/114 / King Abdullah International Medical Research Centre
- Organisation Unit
- School of Health; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99685295902621
- Output Type
- Journal article
Metrics
1 File views/ downloads
20 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Multidisciplinary Sciences
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites