Journal article
Recombinase polymerase amplification: Emergence as a critical molecular technology for rapid, low-resource diagnostics
Expert Review of Molecular Diagnostics, Vol.15(11), pp.1475-1489
2015
Abstract
Isothermal molecular diagnostics are bridging the technology gap between traditional diagnostics and polymerase chain reaction-based methods. These new techniques enable timely and accurate testing, especially in settings where there is a lack of infrastructure to support polymerase chain reaction facilities. Despite this, there is a significant lack of uptake of these technologies in developing countries where they are highly needed. Among these novel isothermal technologies, recombinase polymerase amplification (RPA) holds particular potential for use in developing countries. This rapid nucleic acid amplification approach is fast, highly sensitive and specific, and amenable to countries with a high burden of infectious diseases. Implementation of RPA technology in developing countries is critically required to assess limitations and potentials of the diagnosis of infectious disease, and may help identify impediments that prevent adoption of new molecular technologies in low resource- and low skill settings. This review focuses on approaching diagnosis of infectious disease with RPA.
Details
- Title
- Recombinase polymerase amplification: Emergence as a critical molecular technology for rapid, low-resource diagnostics
- Authors
- Ameh S James (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJoanne Macdonald (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Expert Review of Molecular Diagnostics, Vol.15(11), pp.1475-1489
- Publisher
- Taylor & Francis Ltd.
- Date published
- 2015
- DOI
- 10.1586/14737159.2015.1090877
- ISSN
- 1473-7159
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449380602621
- Output Type
- Journal article
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