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Published VersionCC BY V4.0, Open Access
Journal article
Recombinant luteinizing hormone as a tool towards understanding the reproductive dysfunction in captive Macquarie perch Macquaria australasica
Endangered Species Research, Vol.51, pp.285-291
2023
Abstract
The Macquarie perch (Macquaria australasica) is a critically endangered freshwater fish endemic to the Murray-Darling Basin in Australia. Captive breeding is considered as an essential measure to save the species from extinction, yet its reproductive biology is not fully understood. We produced a recombinant single-chain Macquarie perch luteinizing hormone (rmpLh) in the methylotrophic yeast (Pichia pastoris), confirmed by Western blot analysis and mass spectrometry. We developed and validated a heterologous competitive enzyme-linked immunosorbent assay (ELISA) for M. australasica Lh using antibodies generated against the giant grouper (Epinephelus lanceolatus) Lhb, which is 91% identical to the M. australasica sequence at the amino acid level. Measurement of Lh in plasma samples collected at two timepoints (June vs. August 2020) from captive F1 broodstock did not show significant difference. Plasma Lh in samples from wild fish collected in September 2020, a month prior to the spawning season, were almost 10-fold higher than those of the F1 broodstock. The tools generated here will help understand the reproductive biology of M. australasica and develop reliable assisted reproduction techniques for the species.
Details
- Title
- Recombinant luteinizing hormone as a tool towards understanding the reproductive dysfunction in captive Macquarie perch Macquaria australasica
- Authors
- Josephine Nocillado (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationDean Gilligan (Author) - New South Wales Department of Primary IndustriesStewart Fielder (Author) - New South Wales Department of Primary IndustriesLachlan P Dennis (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTomer Ventura (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationAbigail Elizur (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- Endangered Species Research, Vol.51, pp.285-291
- Publisher
- Inter-Research
- Date published
- 2023
- DOI
- 10.3354/esr01256
- ISSN
- 1613-4796; 1863-5407
- Copyright note
- © The authors 2023. Open Access under Creative Commons by Attribution Licence. Use, distribution and reproduction are unrestricted. Authors and original publication must be credited.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99741698902621
- Output Type
- Journal article
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