Journal article
Real-time PCR detection of pathogenic microorganisms in roof-harvested rainwater in Southeast Queensland, Australia
Applied and Environmental Microbiology, Vol.74(17), pp.5490-5496
2008
Abstract
In this study, the microbiological quality of roof-harvested rainwater was assessed by monitoring the concentrations of Escherichia coli, enterococci, Clostridium perfringens, and Bacteroides spp. in rainwater obtained from tanks in Southeast Queensland, Australia. Samples were also tested using real-time PCR (with SYBR Green I dye) for the presence of potential pathogenic microorganisms. Of the 27 rainwater samples tested, 17 (63%), 21 (78%), 13 (48%), and 24 (89%) were positive for E. coli, enterococci, C. perfringens, and Bacteroides spp., respectively. Of the 27 samples, 11 (41%), 7 (26%), 4 (15%), 3 (11%), and 1 (4%) were PCR positive for the Campylobacter coli ceuE gene, the Legionella pneumophila mip gene, the Aeromonas hydrophila lip gene, the Salmonella invA gene, and the Campylobacter jejuni mapA gene. Of the 21 samples tested, 4 (19%) were positive for the Giardia lamblia β-giardin gene. The binary logistic regression model indicated a positive correlation (P < 0.02) between the presence/absence of enterococci and A. hydrophila. In contrast, the presence/absence of the remaining potential pathogens did not correlate with traditional fecal indicators. The poor correlation between fecal indicators and potential pathogens suggested that fecal indicators may not be adequate to assess the microbiological quality of rainwater and consequent health risk. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
Details
- Title
- Real-time PCR detection of pathogenic microorganisms in roof-harvested rainwater in Southeast Queensland, Australia
- Authors
- Warish Ahmed (Author) - Queensland University of TechnologyF Huygens (Author) - Queensland University of TechnologyA Goonetilleke (Author) - Queensland University of TechnologyTed Gardner (Author) - Queensland University of Technology
- Publication details
- Applied and Environmental Microbiology, Vol.74(17), pp.5490-5496
- Publisher
- American Society for Microbiology
- Date published
- 2008
- DOI
- 10.1128/AEM.00331-08
- ISSN
- 0099-2240
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450582902621
- Output Type
- Journal article
Metrics
304 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites