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Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples - A Veterinary Practitioner’s Perspective536.56 kB
Published VersionCC BY V4.0, Open Access
Journal article
Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner’s Perspective
Pathogens, Vol.10(9), pp.1-9
2021
Abstract
Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.73% positive agreement (PA); 84.75% negative agreement (NA)) overall agreeance for swab samples, and 85.71% (12/14; 80% PA; 88.89% NA) overall agreeance for tissue samples. Out of the 11 total discrepant results, discrepancy was mainly observed in samples (n = 10) with less than 100 copies/µL C. pecorum DNA. While sensitivity could be improved, the simplicity, low cost, and accuracy of detection makes this test amenable for use at point-of-care for detecting C. pecorum in sheep.
Details
- Title
- Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner’s Perspective
- Authors
- Tom Clune (Author) - Murdoch UniversitySusan Anstey (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyVasilli Kasimov (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyCaroline Jacobson (Author) - Murdoch UniversityMartina Jelocnik (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
- Publication details
- Pathogens, Vol.10(9), pp.1-9
- Publisher
- MDPI AG
- Date published
- 2021
- DOI
- 10.3390/pathogens10091157
- ISSN
- 2076-0817
- Copyright note
- Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Grant note
- Lamb necropsies and sample collection was supported with funding from Meat and Livestock Australia (B.AHE.0318). Laboratory work was supported with funding by the Australian Government Department of Agriculture in partnership with Australian Wool Innovation as part of the 2020 Science and Innovation Awards for Young People in Agriculture, Fisheries and Forestry awarded to Tom Clune. Tom Clune was supported with postgraduate scholarships from Meat and Livestock Australia and Sheep Industry Business Innovation (Department of Primary Industries and Regional Development, Western Australia).
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99574708902621
- Output Type
- Journal article
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