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Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification453.78 kB
Published VersionCC BY-NC V4.0, Open Access
Journal article
Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification
JAC - Antimicrobial Resistance, Vol.5(2), pp.1-8
2023
PMID: 36936189
Abstract
Background: Due to their prevalence worldwide, the β lactamases CTXM and plasmid mediated CMY2 are important antimicrobial resistance enzymes in a clinical setting. While culture and PCR based detection methods exist for these targets, they are time consuming and require specialist equipment and trained personnel to carry out. Methods: In this study, three rapid diagnostic single plex and a prototype triplex assay were developed, using recombinase polymerase amplification with lateral flow detection (RPALF), and tested for their sensitivity and specificity using two isolate DNA panels (n = 90 and n = 120 isolates). In addition, the RPALF assays were also tested with a small number of faecal extract samples (n = 18). Results: The RPALF assays were able to detect bla CXTM group 1, bla CTXM group 9 and bla CMY2 type variants with high sensitivity (82.1% to 100%) and specificity (100%) within a short turnaround time (15 to 20 min for amplification and detection). Conclusions: RPALF assays developed in this study have the potential to be used at or close to the point of care, as well as in low resource settings, producing rapid results to support healthcare professionals in their treatment decisions.
Details
- Title
- Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification
- Authors
- Nicole G Ertl (Corresponding Author) - The University of QueenslandAdam D Irwin (Author) - The University of QueenslandJoanne Macdonald (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationMichelle J Bauer (Author) - The University of Queensland, UQ Centre for Clinical Research, Faculty of Medicine, Brisbane, QLD, AustraliaClaire Y T Wang (Author) - Children’s Health Queensland Hospital and Health ServicePatrick N A Harris (Author) - The University of QueenslandClaire Heney (Author) - Pathology QueenslandHosam M Zowawi (Author) - The University of QueenslandDavid M Whiley (Author) - The University of Queensland
- Publication details
- JAC - Antimicrobial Resistance, Vol.5(2), pp.1-8
- Publisher
- Oxford University Press
- Date published
- 2023
- DOI
- 10.1093/jacamr/dlad023
- ISSN
- 2632-1823
- PMID
- 36936189
- Copyright note
- This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
- Grant note
- 50272/ Children’s Hospital Foundation Innovator grant
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99714998902621
- Output Type
- Journal article
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- Infectious Diseases
- Microbiology
- Pharmacology & Pharmacy
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