Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detection

Abdulrahman K. S Ayfan; Joanne Macdonald; Patrick N. A Harris; Claire Heney; David L Paterson; Ella Trembizki; Claire Y. T Wang; David M Whiley; Hosam M Zowawi; Adam D Irwin

doi:10.1007/s10096-021-04267-6

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Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detection

Journal article

Peer reviewed

Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detection

Abdulrahman K. S Ayfan, Joanne Macdonald, Patrick N. A Harris, Claire Heney, David L Paterson, Ella Trembizki, Claire Y. T Wang, David M Whiley, Hosam M Zowawi and Adam D Irwin

European Journal of Clinical Microbiology & Infectious Diseases, Vol.40, pp.2447-2453

2021

DOI: https://doi.org/10.1007/s10096-021-04267-6

PMID: 33974185

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Abstract

Carbapenemase

Lateral flow

VIM

Point-of-care

NDM

Recombinase polymerase amplification

Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect blaNDM-type and blaVIM-type carbapenemase genes using recombinase polymerase amplification (RPA) combined with a lateral flow detection. The tests could provide results in approximately 15 min when using DNA extracts, with limits of detection of 9.2 copies/μl for the blaNDM-type assay and 7.5 copies/μl for blaVIM-type assay, and successfully detected all isolates harbouring the carbapenemase encoding genes in a panel of 57 isolates. These RPA tests may be suitable for use in low-resource settings to tailor rapid implementation of infection control precautions and antibiotic stewardship.

Details

Title: Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow–based detection
Authors: Abdulrahman K. S Ayfan (Author) - University of Queensland
Joanne Macdonald (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - Legacy
Patrick N. A Harris (Author) - University of Queensland
Claire Heney (Author) - Pathology Queensland
David L Paterson (Author) - University of Queensland
Ella Trembizki (Author) - University of Queensland
Claire Y. T Wang (Author) - Queensland Children’s Hospital
David M Whiley (Author) - University of Queensland
Hosam M Zowawi (Author) - University of Queensland
Adam D Irwin (Corresponding Author) - University of Queensland
Publication details: European Journal of Clinical Microbiology & Infectious Diseases, Vol.40, pp.2447-2453
Publisher: Springer
DOI: 10.1007/s10096-021-04267-6
ISSN: 1435-4373
PMID: 33974185
Organisation Unit: Centre for Bioinnovation; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99534608302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Microbiology

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