Rapid and Reliable HPLC Method for the Simultaneous Determination of Dihydroxyacetone, Methylglyoxal and 5-Hydroxymethylfurfural in Leptospermum Honeys

Matthew Pappalardo; Linda Pappalardo; Peter R Brooks

doi:10.1371/journal.pone.0167006

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Rapid and Reliable HPLC Method for the Simultaneous Determination of Dihydroxyacetone, Methylglyoxal and 5-Hydroxymethylfurfural in Leptospermum Honeys

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Rapid and Reliable HPLC Method for the Simultaneous Determination of Dihydroxyacetone, Methylglyoxal and 5-Hydroxymethylfurfural in Leptospermum Honeys

Matthew Pappalardo, Linda Pappalardo and Peter R Brooks

PLoS One, Vol.11(11), e0167006

2016

DOI: https://doi.org/10.1371/journal.pone.0167006

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Abstract

A reliable determination of dihydroxyacetone, methylglyoxal and 5-hydroxymethylfurfural is essential to establishing the commercial value and antimicrobial potential of honeys derived from the Leptospermum species endemic to Australia and New Zealand. We report a robust method for quantitation of all three compounds in a single HPLC run. Honey samples (n = 6) that are derivatized with o-(2,3,4,5,6-Pentafluorobenzyl) hydroxylamine were quantitated against a stable anisole internal standard. Linear regression analysis was performed using calibration standards for each compound (n = 6) and results indicated a high degree of accuracy (R2 = 0.999) for this method. The reliability of some commercial methylglyoxal solutions were found to be questionable. Effective quantitation of methylglyoxal content in honey is critical for researchers and industry, and the use of some commercial standards may bias data. Two accurate methylglyoxal standards are proposed, including a commercial standard and a derivative that can be prepared within the laboratory.

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Title: Rapid and Reliable HPLC Method for the Simultaneous Determination of Dihydroxyacetone, Methylglyoxal and 5-Hydroxymethylfurfural in Leptospermum Honeys
Authors: Matthew Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Linda Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Peter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: PLoS One, Vol.11(11), e0167006; 10
Publisher: Public Library of Science
Date published: 2016
DOI: 10.1371/journal.pone.0167006
ISSN: 1932-6203
Copyright note: Copyright © 2016 Pappalardo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450566602621
Output Type: Journal article

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