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Published VersionCC BY V4.0, Open Access
Journal article
Rapid Diagnostic Tests for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices
Microbiology Spectrum, Vol.11(1), pp.1-12
2023
PMID: 36682882
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Abstract
The efficient and accurate diagnosis of dengue, a major mosquito-borne disease, is of primary importance for clinical care, surveillance, and outbreak control. The identification of specific dengue virus serotype 1 (DENV-1) to DENV-4 can help in understanding the transmission dynamics and spread of dengue disease. The four rapid low-resource serotype-specific dengue tests use a simple sample preparation reagent followed by reverse transcription-isothermal recombinase polymerase amplification (RT-RPA) combined with lateral flow detection (LFD) technology. Results are obtained directly from clinical sample matrices in 35 min, requiring only a heating block and pipettes for liquid handling. In addition, we demonstrate that the rapid sample preparation step inactivates DENV, improving laboratory safety. Human plasma and serum were spiked with DENV, and DENV was detected with analytical sensitivities of 333 to 22,500 median tissue culture infectious doses (TCID50)/mL. The analytical sensitivities in blood were 94,000 to 333,000 TCID50/mL. Analytical specificity testing confirmed that each test could detect multiple serotype-specific strains but did not respond to strains of other serotypes, closely related flaviviruses, or chikungunya virus. Clinical testing on 80 human serum samples demonstrated test specificities of between 94 and 100%, with a DENV-2 test sensitivity of 100%, detecting down to 0.004 PFU/μL, similar to the sensitivity of the PCR test; the other DENV tests detected down to 0.03 to 10.9 PFU/μL. Collectively, our data suggest that some of our rapid dengue serotyping tests provide a potential alternative to conventional labor-intensive RT-quantitative PCR (RT-qPCR) detection, which requires expensive thermal cycling instrumentation, technical expertise, and prolonged testing times. Our tests provide performance and speed without compromising specificity in human plasma and serum and could become promising tools for the detection of high DENV loads in resource-limited settings.
Details
- Title
- Rapid Diagnostic Tests for the Detection of the Four Dengue Virus Serotypes in Clinically Relevant Matrices
- Authors
- Nina Pollak (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationMalin Olsson (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringMadeeha Ahmed (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringJavier Tan (Author) - National Environment AgencyGeorge Lim (Author) - National Environment AgencyYin Xiang Setoh (Author) - National Environmental AgencyJudith Chui Ching Wong (Author) - National Environment AgencyYee Ling Lai (Author) - National Environment AgencyJody Hobson-Peters (Author) - The University of QueenslandJoanne Macdonald (Author) - BioCifer Pty LtdDavid McMillan (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
- Publication details
- Microbiology Spectrum, Vol.11(1), pp.1-12
- Publisher
- American Society for Microbiology
- Date published
- 2023
- DOI
- 10.1128/spectrum.02796-22
- ISSN
- 2165-0497
- PMID
- 36682882
- Copyright note
- © 2023 Pollak et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99701597102621
- Output Type
- Journal article
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