Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method

P Gyawali; Jatinder P S Sidhu; Warish Ahmed; P Jagals; S Toze

doi:10.1016/j.exppara.2015.08.009

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Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method

Journal article

Open access

Peer reviewed

Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method

P Gyawali, Jatinder P S Sidhu, Warish Ahmed, P Jagals and S Toze

Experimental Parasitology, Vol.159, pp.5-12

2015

DOI: https://doi.org/10.1016/j.exppara.2015.08.009

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Abstract

real-time PCR

helminth

hookworm

wastewater

sludge

health risk assessment

The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. In this study, a real-time PCR method was developed for the rapid, sensitive and specific detection of canine hookworm (Ancylostoma caninum) ova from wastewater matrices. A. caninum was chosen because of its morphological similarity to the human hookworm (Ancylostoma duodenale and Necator americanus). The newly developed PCR method has low detection sensitivity with the ability to detect less than one A. caninum ova from 1 L of secondary treated wastewater at the mean threshold cycle (CT) values ranging from 30.1-34.3. The method is also able to detect four A. caninum ova from 1 L of raw wastewater and from ∼ 4 gm of treated sludge with mean CT values ranging from 35.6-39.8 and 39.8-39.9, respectively. The better detection sensitivity obtained for secondary treated wastewater compared to raw wastewater and sludge samples could be attributed to sample turbidity. The proposed method appears to be rapid, sensitive and specific compared to traditional methods and has potential to aid in the public health risk assessment associated with land application of wastewater matrices. Furthermore, the method can be adapted to detect other helminth ova of interest from wastewater matrices.

Details

Title: Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method
Authors: P Gyawali (Author) - CSIRO Land and Water
Jatinder P S Sidhu (Author) - University of Queensland
Warish Ahmed (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
P Jagals (Author) - University of Queensland
S Toze (Author) - University of Queensland
Publication details: Experimental Parasitology, Vol.159, pp.5-12
Publisher: Academic Press
Date published: 2015
DOI: 10.1016/j.exppara.2015.08.009
ISSN: 0014-4894
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449477702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Parasitology

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