Journal article
Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method
Experimental Parasitology, Vol.159, pp.5-12
2015
Abstract
The risk of human hookworm infections from land application of wastewater matrices could be high in regions with high hookworm prevalence. A rapid, sensitive and specific hookworm detection method from wastewater matrices is required in order to assess human health risks. Currently available methods used to identify hookworm ova to the species level are time consuming and lack accuracy. In this study, a real-time PCR method was developed for the rapid, sensitive and specific detection of canine hookworm (Ancylostoma caninum) ova from wastewater matrices. A. caninum was chosen because of its morphological similarity to the human hookworm (Ancylostoma duodenale and Necator americanus). The newly developed PCR method has low detection sensitivity with the ability to detect less than one A. caninum ova from 1 L of secondary treated wastewater at the mean threshold cycle (CT) values ranging from 30.1-34.3. The method is also able to detect four A. caninum ova from 1 L of raw wastewater and from ∼ 4 gm of treated sludge with mean CT values ranging from 35.6-39.8 and 39.8-39.9, respectively. The better detection sensitivity obtained for secondary treated wastewater compared to raw wastewater and sludge samples could be attributed to sample turbidity. The proposed method appears to be rapid, sensitive and specific compared to traditional methods and has potential to aid in the public health risk assessment associated with land application of wastewater matrices. Furthermore, the method can be adapted to detect other helminth ova of interest from wastewater matrices.
Details
- Title
- Rapid Concentration and Sensitive Detection of Hookworm Ova from Wastewater Matrices Using a Real-Time PCR Method
- Authors
- P Gyawali (Author) - CSIRO Land and WaterJatinder P S Sidhu (Author) - University of QueenslandWarish Ahmed (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringP Jagals (Author) - University of QueenslandS Toze (Author) - University of Queensland
- Publication details
- Experimental Parasitology, Vol.159, pp.5-12
- Publisher
- Academic Press
- Date published
- 2015
- DOI
- 10.1016/j.exppara.2015.08.009
- ISSN
- 0014-4894; 0014-4894
- Copyright note
- Copyright © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99449477702621
- Output Type
- Journal article
- Research Statement
- false
Metrics
97 File views/ downloads
563 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Parasitology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites