Raman Microscopy and X-ray Diffraction, a Combined Study of Fibrillin-rich Microfibrillar Elasticity

J Louise Haston; Soren B Engelsen; Manfred Roessle; John Clarkson; Ewan W Blanch; Clair Baldock; Cay M Kielty; Tim J Wess

doi:10.1074/jbc.M212854200

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$Raman Microscopy and X-ray Diffraction, a Combined Study of Fibrillin-rich Microfibrillar Elasticity$

Journal article

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Raman Microscopy and X-ray Diffraction, a Combined Study of Fibrillin-rich Microfibrillar Elasticity

J Louise Haston, Soren B Engelsen, Manfred Roessle, John Clarkson, Ewan W Blanch, Clair Baldock, Cay M Kielty and Tim J Wess

Journal of Biological Chemistry, Vol.278(42), pp.41189-41197

2003

DOI: https://doi.org/10.1074/jbc.M212854200

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Abstract

Fibrillin-rich microfibrils are essential elastic structures contained within the extracellular matrix of a wide variety of connective tissues. Microfibrils are characterized as beaded filamentous structures with a variable axial periodicity (average 56 nm in the untensioned state); however, the basis of their elasticity remains unknown. This study used a combination of small angle x-ray scattering and Raman microscopy to investigate further the packing of microfibrils within the intact tissue and to determine the role of molecular reorganization in the elasticity of these microfibrils. The application of relatively small strains produced no overall change in either molecular or macromolecular microfibrillar structure. In contrast, the application of larger tissue extensions (up to 150%) resulted in a markedly different structure, as observed by both Raman microscopy and small angle x-ray scattering. These changes occurred at different levels of architecture and are interpreted as ranging from alterations in peptide bond conformation to domain rearrangement. This study demonstrates the importance of molecular elasticity in the mechanical properties of fibrillin-rich microfibrils in the intact tissue.

Details

Title: Raman Microscopy and X-ray Diffraction, a Combined Study of Fibrillin-rich Microfibrillar Elasticity
Authors: J Louise Haston (Author) - University of Stirling, United Kingdom
Soren B Engelsen (Author) - Royal Veterinary and Agricultural University, Denmark
Manfred Roessle (Author) - European Synchrotron Radiation Facility, France
John Clarkson (Author) - University of Glasgow, United Kingdom
Ewan W Blanch (Author) - University of Manchester, United Kingdom
Clair Baldock (Author) - University of Manchester, United Kingdom
Cay M Kielty (Author) - University of Manchester, United Kingdom
Tim J Wess (Author) - University of Stirling, United Kingdom
Publication details: Journal of Biological Chemistry, Vol.278(42), pp.41189-41197
Publisher: American Society for Biochemistry and Molecular Biology, Inc.
Date published: 2003
DOI: 10.1074/jbc.M212854200
ISSN: 0021-9258
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451225202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology

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