Quantitative PCR assay of sewage-associated bacteroides markers to assess sewage pollution in an urban lake in Dhaka, Bangladesh

Warish Ahmed; R Yusuf; I Hasan; A Goonetilleke; Ted Gardner

doi:10.1139/W10-070

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Quantitative PCR assay of sewage-associated bacteroides markers to assess sewage pollution in an urban lake in Dhaka, Bangladesh

Journal article

Open access

Peer reviewed

Quantitative PCR assay of sewage-associated bacteroides markers to assess sewage pollution in an urban lake in Dhaka, Bangladesh

Warish Ahmed, R Yusuf, I Hasan, A Goonetilleke and Ted Gardner

Canadian Journal of Microbiology, Vol.56(10), pp.838-845

2010

DOI: https://doi.org/10.1139/W10-070

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Abstract

sewage pollution

fecal indicator bacteria

microbial source tracking

host-specific molecular markers

public health risk

This paper aimed to assess the magnitude of sewage pollution in an urban lake in Dhaka, Bangladesh, by using quantitative PCR of sewage-associated Bacteroides HF183 markers. PCR was also used for the quantitative detection of ruminant wastewater-associated CF128 markers along with the enumeration of traditional fecal indicator bacteria, namely enterococci. The number of enterococci in lake water samples ranged from 1.1 × 104 to 1.9 × 105 colony-forming units/ 100 mL water. From the 20 water samples tested, 14 (70%) and 7 (35%) were PCR positive for HF183 and CF128 markers, respectively. The numbers of HF183 and CF128 markers in lake water samples were 3.9 × 104 to 6.3 × 107 and 9.3 × 103 to 6.3 × 105 genomic units/100 mL water, respectively. The high numbers of enterococci and HF183 markers are indicative of sewage pollution and potential health risks to those who use the lake water for nonpotable purposes such as bathing and washing clothes. This is the first study that investigated the presence of microbial source tracking markers in Dhaka, Bangladesh, where diarrhoeal disease is one of the major causes of childhood mortality. The molecular assay used in this study can provide valuable information on the extent of sewage pollution, thus facilitating the development of robust strategies to minimize potential health risks.

Details

Title: Quantitative PCR assay of sewage-associated bacteroides markers to assess sewage pollution in an urban lake in Dhaka, Bangladesh
Authors: Warish Ahmed (Author) - Queensland University of Technology
R Yusuf (Author) - Independent University of Bangladesh, Bangladesh
I Hasan (Author) - Rajshahi University, Bangladesh
A Goonetilleke (Author) - Queensland University of Technology
Ted Gardner (Author) - Queensland University of Technology
Publication details: Canadian Journal of Microbiology, Vol.56(10), pp.838-845
Publisher: N R C Research Press
Date published: 2010
DOI: 10.1139/W10-070
ISSN: 0008-4166
Copyright note: Copyright © 2010 The Authors. The Author's Accepted Version is reproduced with permission. The final definitive version is available from http://dx.doi.org/10.1139/W10-070
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450439202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology

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