Quantitation of Dihydroxyacetone in Australian Leptospermum Nectar via High-Performance Liquid Chromatography

Amanda M Norton; Leah N McKenzie; Peter R Brooks; Linda Pappalardo

doi:10.1021/acs.jafc.5b01930

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Quantitation of Dihydroxyacetone in Australian Leptospermum Nectar via High-Performance Liquid Chromatography

Journal article

Peer reviewed

Quantitation of Dihydroxyacetone in Australian Leptospermum Nectar via High-Performance Liquid Chromatography

Amanda M Norton, Leah N McKenzie, Peter R Brooks and Linda Pappalardo

Journal of Agricultural and Food Chemistry, Vol.63(29), pp.6513-6517

2015

DOI: https://doi.org/10.1021/acs.jafc.5b01930

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Abstract

dihydroxyacetone

Leptospermum

nectar

Australia

high-performance liquid chromatography

honey

The nonperoxide antibacterial activity of New Zealand maÌ…nuka honey originates from dihydroxyacetone (DHA) within Leptospermum scoparium nectar. This study determined if DHA was present within the nectar of four Australian Leptospermum species: L. laevigatum, L. polygalifolium, L. trinervium, and L. whitei. A rapid and convenient new method was developed, which quantitated DHA/sugar ratios (ppm). The DHA and sugars were derivatized with o-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine hydrochloride and analyzed via RP-HPLC with diode array detection at two wavelengths (200 and 243 nm). DHA was detected in all L. whitei and L. polygalifolium samples, where DHA/sugar ratios ranged from 10169 to 24199 ppm and from 9321 to 20174 ppm, respectively. DHA was undetected in any of the L. laevigatum and L. trinervium samples, and nectar activity was <100 ppm. The results of this study have implications for the Australian beekeeping industry, as the findings indicated that not all species of Leptospermum will produce active honey.

Details

Title: Quantitation of Dihydroxyacetone in Australian Leptospermum Nectar via High-Performance Liquid Chromatography
Authors: Amanda M Norton (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Leah N McKenzie (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Peter R Brooks (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Linda Pappalardo (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Journal of Agricultural and Food Chemistry, Vol.63(29), pp.6513-6517
Publisher: American Chemical Society
Date published: 2015
DOI: 10.1021/acs.jafc.5b01930
ISSN: 0021-8561
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; Academic Support Unit; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449306702621
Output Type: Journal article

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