Journal article
Quantifying the binding between proteins and open chromatin-like DNA sequences with gold nanorods
Chemical Communications, Vol.55(100), pp.15041-15044
2019
Abstract
The binding of transcription factors to DNA is one of the main mechanisms in gene regulation. While transcription factors frequently bind to unwrapped long DNA sequences known as open chromatin structures, most bioassays that study protein-DNA binding rely on short oligonucleotide probes. In this work, we develop a gold nanorod-based colorimetric assay for the binding of transcription factors to DNA in long open chromatin-like structures. After the determination of the binding affinity and stoichiometry, we explored the effect of the probe length on the assay performance and compared it to other established techniques.
Details
- Title
- Quantifying the binding between proteins and open chromatin-like DNA sequences with gold nanorods
- Authors
- Roger M Pallares (Author)Nguyen Thi Kim Thanh (Author)Xiao Du Su (Author) - University of the Sunshine Coast - School of Science & Engineering
- Publication details
- Chemical Communications, Vol.55(100), pp.15041-15044
- Publisher
- Royal Society of Chemistry (R S C) Publications
- Date published
- 2019
- DOI
- 10.1039/C9CC07511A
- ISSN
- 1359-7345
- Copyright note
- Copyright © 2019 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99450943502621
- Output Type
- Journal article
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