Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: Oxytetracycline production by Streptomyces rimosus

A P Taylor; K S Finnie; John R Bartlett; P J Holden

doi:10.1007/s10529-004-3740-8

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Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: Oxytetracycline production by Streptomyces rimosus

Journal article

Peer reviewed

Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: Oxytetracycline production by Streptomyces rimosus

A P Taylor, K S Finnie, John R Bartlett and P J Holden

Biotechnology Letters, Vol.26(22), pp.1707-1711

2004

DOI: https://doi.org/10.1007/s10529-004-3740-8

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Abstract

Biological Sciences

Engineering

Technology

bioencapsulation

oxytetracycline

silica gel

streptomyces rimosus

UV-sterilisation

A technique was developed for differentiating the activity of microbes solely within sol gels by using the contribution of biomass outgrowth. Streptomyces rimosus was immobilised in colloidal silica gels and biomass growth, oxytetracycline synthesis, pH and carbohydrate consumption were compared for UV surface-sterilised gels, untreated gels, and liquid cultures. Absolute and biomass specific oxytetracycline yields were higher for non-sterile gels than for liquid culture. Biomass solely within colloidal silica gels (1.7 mg ml -1), and gels obtained from colloidal silica modified by addition of larger silica particles (1.2 mg ml -1) yielded 27 and 21 μg ml -1 oxytetracycline compared with 97 and 104 μg ml -1 for unsterilised gels (3.6 and 5.2 mg ml -1 biomass) displaying outgrowth. It was therefore apparent that biomass and antibiotic production within the gels was limited and that optimisation requires gel modification.

Details

Title: Quantification of the contribution of surface outgrowth to biocatalysis in sol-gels: Oxytetracycline production by Streptomyces rimosus
Authors: A P Taylor (Author) - Australian Nuclear Science and Technology Organisation Materials Department
K S Finnie (Author) - Australian Nuclear Science and Technology Organisation Materials Department
John R Bartlett (Author) - Australian Nuclear Science and Technology Organisation Materials Department
P J Holden (Author) - Australian Nuclear Science and Technology Organisation Materials Department
Publication details: Biotechnology Letters, Vol.26(22), pp.1707-1711
Publisher: Springer Netherlands
Date published: 2004
DOI: 10.1007/s10529-004-3740-8
ISSN: 0141-5492
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450062402621
Output Type: Journal article

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Web Of Science research areas: Biotechnology & Applied Microbiology