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Pyrosequencing-based characterization of gastrointestinal bacteria of Atlantic salmon (Salmo salar L.) within a commercial mariculture system
Journal article   Open access   Peer reviewed

Pyrosequencing-based characterization of gastrointestinal bacteria of Atlantic salmon (Salmo salar L.) within a commercial mariculture system

Kamarul Zaman Zarkasi, Guy C J Abell, Richard S Taylor, Christina Neuman, Eva Hatje, Mark L Tamplin, Mohammad Katouli and John P Bowman
Journal of Applied Microbiology, Vol.117(1), pp.18-27
2014
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https://doi.org/10.1111/jam.12514View
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Abstract

atlantic salmon intestinal bacteria 16S rRNA gene NGS pyrosequencing season
Aims: The relationship of Atlantic salmon gastrointestinal tract bacteria to environmental factors, in particular water temperature within a commercial mariculture system, was investigated. Methods and Results: Salmon gastrointestinal tract bacterial communities of commercially farmed in south-eastern Tasmania was analysed, over a 13 month period across a standard commercial production farm cycle, using 454 16S rRNA-based pyrosequencing. Faecal bacterial communities were highly dynamic but largely similar between randomly selected fish. In post-smolt, the faecal bacteria population was dominated by Gram-positive fermentative bacteria, however by mid-summer members of the family Vibrionaceae predominated. As fish progressed towards harvest, a range of different bacterial genera became more prominent corresponding to a decline in Vibrionaceae. The sampled fish were fed two different commercial diet series with slightly different protein, lipid and digestible energy level, however the effect of these differences was minimal. Conclusions: The overall data demonstrated dynamic hind gut communities in salmon that were related to season and fish growth phases but were less influence by differences in commercial diets used routinely within the farm system studied.

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Biotechnology & Applied Microbiology
Microbiology
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