Journal article
Pyocyanin induces systemic oxidative stress, inflammation and behavioral changes in vivo
Toxicology Mechanisms and Methods, Vol.28(6), pp.410-414
2018
PMID: 29338525
Abstract
Pyocyanin (PCN) is a virulence factor secreted by Pseudomonas aeruginosa (P. aeruginosa) that has been shown to have numerous toxic effects in both in vitro and in vivo studies. Such toxicities include pro-inflammatory and pro-oxidant mediated responses. It is hypothesized that PCN can cross biological membranes and reach the systemic circulation, but no previous studies have investigated this. The aim of this study was, therefore, to quantify PCN in plasma and assess if systemic responses were occurring after localized intranasal administration in C57BL/6J mice. This was achieved through the plasma quantification of PCN and assessment of changes to behavior using two commonly used tests, the forced swimming test and the open field test. Furthermore, evidence of systemic oxidative stress and inflammation was measured using malondialdehyde (MDA) and TNF-alpha post PCN exposure. PCN was found to cross into systemic circulation but in a variable manner. Furthermore, significant increases in plasma TNF-alpha and MDA (both p < 0.001) were observed along with changes in behavior indicative of systemic inflammatory responses.
Details
- Title
- Pyocyanin induces systemic oxidative stress, inflammation and behavioral changes in vivo
- Authors
- Devinder Arora (Author) - Griffith UniversitySusan Hall (Author) - Griffith UniversityShailendra Anoopkumar-Dukie (Author) - Griffith UniversityRachel Morrison (Author) - Griffith UniversityAmelia McFarland (Author) - Griffith UniversityAnthony V. Perkins (Author) - Griffith UniversityAndrew K. Davey (Author) - Griffith UniversityGary D. Grant (Author) - Griffith University
- Publication details
- Toxicology Mechanisms and Methods, Vol.28(6), pp.410-414
- Publisher
- Taylor & Francis Inc.
- DOI
- 10.1080/15376516.2018.1429038
- ISSN
- 1537-6524
- PMID
- 29338525
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health
- Language
- English
- Record Identifier
- 99685198402621
- Output Type
- Journal article
Metrics
10 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Toxicology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites