Pyocyanin-induced toxicity in A549 respiratory cells is causally linked to oxidative stress

Lee S. Gloyne; Gary D. Grant; Anthony V. Perkins; Katie L. Powell; Catherine M. McDermott; Peter V. Johnson; Gregory J. Anderson; Milton Kiefel; Shailendra Anoopkumar-Dukie

doi:10.1016/j.tiv.2011.05.004

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Pyocyanin-induced toxicity in A549 respiratory cells is causally linked to oxidative stress

Journal article

Peer reviewed

Pyocyanin-induced toxicity in A549 respiratory cells is causally linked to oxidative stress

Lee S. Gloyne, Gary D. Grant, Anthony V. Perkins, Katie L. Powell, Catherine M. McDermott, Peter V. Johnson, Gregory J. Anderson, Milton Kiefel and Shailendra Anoopkumar-Dukie

Toxicology in Vitro, Vol.25(7), pp.1353-1358

2011

DOI: https://doi.org/10.1016/j.tiv.2011.05.004

PMID: 21596130

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Abstract

A549 respiratory epithelial cells

Glutathione

Oxidative stress

Pseudomonas aeruginosa

Pyocyanin

Pyocyanin, a virulence factor produced by Pseudomonas aeruginosa, has many damaging effects on mammalian cells. Several lines of evidence suggest that this damage is primarily mediated by its ability to generate ROS and deplete host antioxidant defence mechanisms. However, a causal role for oxidative stress has not yet been demonstrated conclusively. Parallel measures of ROS production, antioxidant levels and cytotoxicity provide convincing evidence that pyocyanin-induced cytotoxicity in A549 respiratory cells is mediated by acute ROS production and subsequent oxidative stress. Pyocyanin increased ROS levels in A549 cells as measured by the fluorescent H2O2 probes Amplex Red and DCFH-DA. These effects were attenuated by the antioxidant N-acetylcysteine. Furthermore, pyocyanin-induced depletion of intracellular GSH levels 24 h after exposure was also prevented by pre-treatment of cells with NAC. Under these conditions, NAC protected cells against pyocyanin-induced cytotoxicity as measured by resazurin reduction to resorufin and viable cell counts, strongly supporting a causal role for oxidative stress. Finally, we also show that pyocyanin-induced activation of the immune and inflammatory transcription factor NF-κB in A549 cells is likely mediated by increased ROS. This increased understanding of mechanisms underlying pyocyanin-induced cytotoxicity may ultimately lead to better strategies for reducing the virulence associated with chronic P. aeruginosa infection.

Details

Title: Pyocyanin-induced toxicity in A549 respiratory cells is causally linked to oxidative stress
Authors: Lee S. Gloyne (Author) - Griffith University
Gary D. Grant (Author) - Griffith University
Anthony V. Perkins (Author) - Griffith University
Katie L. Powell (Author) - Griffith University
Catherine M. McDermott (Author) - Bond University
Peter V. Johnson (Author) - Bond University
Gregory J. Anderson (Author) - QIMR Berghofer Medical Research Institute
Milton Kiefel (Author) - Griffith University
Shailendra Anoopkumar-Dukie (Corresponding Author) - Griffith University
Publication details: Toxicology in Vitro, Vol.25(7), pp.1353-1358
Publisher: Elsevier Ltd
DOI: 10.1016/j.tiv.2011.05.004
ISSN: 1879-3177
PMID: 21596130
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health
Language: English
Record Identifier: 99685195302621
Output Type: Journal article

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Web Of Science research areas: Toxicology

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