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Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus1.40 MB
Published VersionCC BY V4.0, Open Access
Journal article
Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus
Biology, Vol.11(3), pp.1-12
2022
PMCID: PMC8945326
PMID: 35336837
Abstract
Assassin bugs use their salivary venoms for various purposes, including defense, prey paralyzation, and extra-oral digestion, but the mechanisms underlying the functional complexity of the venom remain largely unclear. Since venom glands are composed of several chambers, it is suggested that individual chambers may be specialized to produce chemically distinct venoms to exert different functions. The current study assesses this hypothesis by performing toxicity assays and transcriptomic and proteomic analysis on components from three major venom gland chambers including the anterior main gland (AMG), the posterior main gland (PMG), and the accessory gland (AG) of the assassin bug. Proteotranscriptomic analysis reveals that AMG and PMG extracts are rich in hemolytic proteins and serine proteases, respectively, whereas transferrin and apolipophorin are dominant in the AG. Toxicity assays reveal that secretions from different gland chambers have distinct effects on the prey, with that from AG compromising prey mobility, that from PMG causing prey death and liquifying the corpse, and that from AMG showing no significant physiological effects. Our study reveals a functional cooperation among venom gland chambers of assassin bugs and provides new insights into physiological adaptations to venom-based predation and defense in venomous predatory bugs.
Details
- Title
- Proteotranscriptomic Analysis and Toxicity Assay Suggest the Functional Distinction between Venom Gland Chambers in Twin-Spotted Assassin Bug, Platymeris biguttatus
- Authors
- Fanding Gao (Author) - China Agricultural UniversityLi Tian (Author) - China Agricultural UniversityXinyu Li (Author) - China Agricultural UniversityYinqiao Zhang (Author) - China Agricultural UniversityTianfang Wang (Author) - University of the Sunshine Coast, Queensland, GeneCology Research Centre - LegacyLing Ma (Author) - China Agricultural UniversityFan Song (Author) - China Agricultural UniversityWanzhi Cai (Author) - China Agricultural UniversityHu Li (Author) - China Agricultural University
- Publication details
- Biology, Vol.11(3), pp.1-12
- Publisher
- MDPI AG
- Date published
- 2022
- DOI
- 10.3390/biology11030464
- ISSN
- 2079-7737
- PMID
- 35336837; PMC8945326
- Copyright note
- Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99622937002621
- Output Type
- Journal article
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