Proteomics of sperm duct and spermatophore from the ornate spiny lobster, Panulirus ornatus: identifying the precursor of adipokinetic hormone/corazonin-like peptide (ACP)

Susan Glendinning; Tianfang Wang; Malin Olsson; Trong D Tran; David McMillan; Quinn P Fitzgibbon; Gregory G Smith; Tomer Ventura

doi:10.1016/j.cbpb.2026.111286

Back

Proteomics of sperm duct and spermatophore from the ornate spiny lobster, Panulirus ornatus: identifying the precursor of adipokinetic hormone/corazonin-like peptide (ACP)

Journal article

Open access

Peer reviewed

Proteomics of sperm duct and spermatophore from the ornate spiny lobster, Panulirus ornatus: identifying the precursor of adipokinetic hormone/corazonin-like peptide (ACP)

Susan Glendinning, Tianfang Wang, Malin Olsson, Trong D Tran, David McMillan, Quinn P Fitzgibbon, Gregory G Smith and Tomer Ventura

Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology, Vol.287, pp.1-11

2027

DOI: https://doi.org/10.1016/j.cbpb.2026.111286

PMID: 42772664

Files and links (1)

pdf

Proteomics of sperm duct and spermatophore from the ornate spiny lobster, Panulirus ornatus: identifying the precursor of adipokinetic hormone/corazonin-like peptide (ACP)6.97 MBDownload View

Published Version Open Access CC BY V4.0

Abstract

Proteome

Reproduction

Antioxidant

Adipokinetic hormone/corazonin-like peptide

Crustacean

The adipokinetic hormone/corazonin-like peptide (ACP) functions as a hormone in many arthropod species. The ACP-encoding transcript is expressed in the neural tissues and sperm duct of the ornate spiny lobster, Panulirus ornatus, a species of importance to the aquaculture industry. In this study, the presence of the ACP precursor peptide was verified in the medial sperm duct and spermatophore of P. ornatus through peptide fragments identified by proteomic analysis. We examined the proteome of three tissues: the medial sperm duct, its spermatophoric contents, and a deposited ‘tarspot’, identifying other proteins involved with multiple functions including immune response, amelioration of oxidative stress, antimicrobial action, energy usage, and muscle function. To understand a potential role for ACP precursor peptides in these tissues, a screen targeting nine bacterial strains was carried out however no antimicrobial activity was found for ACP or related peptides. A 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to identify potential mild antioxidant activity in a peptide from the ACP precursor providing a putative function in the sperm duct. The histology of the medial sperm duct of P. ornatus, together with the protein constituents of the medial sperm duct, its contents, and tarspot are presented to provide a greater understanding of the male reproductive system of the ornate spiny lobster.

Details

Title: Proteomics of sperm duct and spermatophore from the ornate spiny lobster, Panulirus ornatus: identifying the precursor of adipokinetic hormone/corazonin-like peptide (ACP)
Authors: Susan Glendinning (Corresponding Author) - University of the Sunshine Coast
Tianfang Wang - University of the Sunshine Coast
Malin Olsson - University of the Sunshine Coast
Trong D Tran - University of the Sunshine Coast
David McMillan - University of the Sunshine Coast
Quinn P Fitzgibbon - University of Tasmania
Gregory G Smith - University of Tasmania
Tomer Ventura - University of the Sunshine Coast
Publication details: Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology, Vol.287, pp.1-11
Publisher: lsevier Inc.
Date published: 2027
DOI: 10.1016/j.cbpb.2026.111286
ISSN: 1879-1107
PMID: 42772664
Data Availability: Data will be made available on request.
Grants: The ARC Research Hub for Sustainable Lobster Aquaculture, IH190100014, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 991262582602621
Output Type: Journal article

Metrics

1 Record Views