Journal article
Proteomic analysis of the venom and venom sac of the woodwasp, Sirex noctilio - towards understanding its biological impact
Journal of Proteomics, Vol.146, pp.195-206
2016
Abstract
The European horntail woodwasp, Sirex noctilio, is an invasive insect that attacks conifer hosts, particularly Pinus species. Venom injected by female S. noctilio, together with its symbiotic fungus, damages the normal physiology of Pinus, leading to death of the tree. To identify the proteinaceous components in the venom and uncover the interplay between venom proteins and tree proteins, clarification of the overall profile of proteins produced in the venom gland apparatus was carried out in this work. The venom sac proteome utilised in-solution digested in either a natural or deglycosylated state, prior to nanoHPLC LTQ-Orbitrap under CID/ETD mode. Here, we report the identification of 1454 and 1225 proteins in venom and sac, respectively, with 410 mutual proteins. Approximately 90 proteins were predicted to be secretory, of which 8 are have features characteristic of toxins. Chemosensory binding proteins were also identified. Gene ontology and KEGG pathway analysis were employed to predict the protein functions and biological pathways in venom and sac. Protein-protein interaction (PPI) analysis suggested that one-step responses represent the majority of the Sirex-Pinus PPIs, and the proteins representing network hub nodes could be of importance for the development of pest management strategies.
Details
- Title
- Proteomic analysis of the venom and venom sac of the woodwasp, Sirex noctilio - towards understanding its biological impact
- Authors
- Tianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMin Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringBronwyn Rotgans (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringGuoying Ni (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringJeffrey F D Dean (Author) - University of Georgia, United StatesHelen F Nahrung (Author) - University of the Sunshine Coast - Faculty of Arts, Business and LawScott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Journal of Proteomics, Vol.146, pp.195-206
- Publisher
- Elsevier BV
- Date published
- 2016
- DOI
- 10.1016/j.jprot.2016.07.002
- ISSN
- 1874-3919
- Copyright note
- Copyright © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- Faculty of Science, Health, Education and Engineering; School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Forest Industries Research Centre; School of Science, Technology and Engineering; Forest Research Institute; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451299502621
- Output Type
- Journal article
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