Files and links (2)
Published VersionCC BY V4.0, Open Access
Journal article
Proteomic analysis of anti-MRSA activity of caerin 1.1/1.9 in a murine skin infection model and their in vitro anti-biofilm effects against Acinetobacter baumannii
Microbiology Spectrum, Vol.11(6), pp.1-16
2023
Abstract
Caerin 1 is a family of host defense peptides with antimicrobial properties originally isolated from the Australian tree frog. Both caerin 1.1 and caerin 1.9 have been demonstrated to inhibit infections caused by multiple antibiotic-resistant bacteria, both in vitro and in vivo. In this current study, we conducted a comparison of the minimum inhibitory concentrations of caerin 1.1 and caerin 1.9 (referred to as caerin 1.1/1.9) with commonly used antibiotics against various bacteria, including Staphylococcus aureus, Copper-Green Pseudomonas aeruginosa, Acinetobacter baumannii, and Streptococcus haemolyticus. Our findings reveal that caerin 1.1/1.9 not only prevent the formation of biofilms by A. baumannii but also exhibit a therapeutic effect on established biofilms. Furthermore, our study demonstrates that caerin 1.1/1.9 significantly inhibit the growth of methicillin-resistant Staphylococcus aureus (MRSA) strains in a murine skin infection model. A quantitative proteomic analysis conducted as part of our study indicates that caerin 1.1/1.9 primarily activate oxidative phosphorylation pathways, along with several pathways associated with tissue repair and growth. These findings were observed in comparison to untreated tissues that were infected with MRSA in mice. In summary, our research outcomes suggest that caerin 1.1/1.9 exhibit the potential to serve as promising drug candidates for treating complicated antibiotic-resistant bacterial infections in humans. IMPORTANCE Caerin 1.1 and caerin 1.9, natural antimicrobial peptides derived from tree frogs, have demonstrated the ability to inhibit the growth of antibiotic-resistant bacteria, comparable to certain widely used antibiotics. Additionally, these peptides exhibit the capacity to prevent or treat biofilms formed by bacteria in conjunction with bodily components. The mechanisms underlying their antibacterial effects were investigated through a mouse model of bacterial skin infection, utilizing proteomic analysis as a technological approach.
Details
- Title
- Proteomic analysis of anti-MRSA activity of caerin 1.1/1.9 in a murine skin infection model and their in vitro anti-biofilm effects against Acinetobacter baumannii
- Authors
- Pingping Zhang (Author) - First People's Hospital of FoshanShuxian Tang (Author) - First People's Hospital of FoshanQuanlan Fu (Author) - Guizhou UniversityYuandong Luo (Author) - Guizhou UniversityJunjie Li (Author) - Guiyang Medical UniversityZhu Chen (Author) - Guiyang Medical UniversityHejie Li (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationGuoying Ni (Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationTianfang Wang (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for BioinnovationGuoqiang Chen (Corresponding Author) - First People's Hospital of FoshanXiaosong Liu (Corresponding Author) - First People's Hospital of Foshan
- Publication details
- Microbiology Spectrum, Vol.11(6), pp.1-16
- Publisher
- American Society for Microbiology
- Date published
- 2023
- DOI
- 10.1128/spectrum.04520-22
- ISSN
- 2165-0497
- Copyright note
- This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
- Data Availability
- The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE(38) partner repository with the dataset identifier PXD044425.
- Grant note
- 2015AG1003 / Foshan Municipal Government 2016A020213001 / GDSTC | Basic and Applied Basic Research Foundation of Guangdong Province 2020A1515010855 / MOST | National Natural Science Foundation of China (NSFC)
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99971192302621
- Output Type
- Journal article
Metrics
25 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Microbiology
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites