Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis thaliana Identifies Major Latex Protein Family Members Critical for Normal Development

Celso Gaspar Litholdo, Jr; Benjamin L Parker; Andrew Eamens; Martin R. Larsen; Stuart J. Cordwell; Peter M. Waterhouse

doi:10.1074/mcp.M115.053124

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Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis thaliana Identifies Major Latex Protein Family Members Critical for Normal Development

Journal article

Peer reviewed

Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis thaliana Identifies Major Latex Protein Family Members Critical for Normal Development

Celso Gaspar Litholdo, Jr, Benjamin L Parker, Andrew Eamens, Martin R. Larsen, Stuart J. Cordwell and Peter M. Waterhouse

Molecular & Cellular Proteomics, Vol.15(6), pp.2033-2047

2016

DOI: https://doi.org/10.1074/mcp.M115.053124

PMCID: PMC5083100

PMID: 27067051

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Abstract

Expression of the F-Box protein Leaf Curling Responsiveness (LCR) is regulated by microRNA, miR394, and alterations to this interplay in Arabidopsis thaliana produce defects in leaf polarity and shoot apical meristem organization. Although the miR394-LCR node has been documented in Arabidopsis, the identification of proteins targeted by LCR F-box itself has proven problematic. Here, a proteomic analysis of shoot apices from plants with altered LCR levels identified a member of the Latex Protein (MLP) family gene as a potential LCR F-box target. Bioinformatic and molecular analyses also suggested that other MLP family members are likely to be targets for this post-translational regulation. Direct interaction between LCR F-Box and MLP423 was validated. Additional MLP members had reduction in protein accumulation, in varying degrees, mediated by LCR F-Box. Transgenic Arabidopsis lines, in which MLP28 expression was reduced through an artificial miRNA technology, displayed severe developmental defects, including changes in leaf patterning and morphology, shoot apex defects, and eventual premature death. These phenotypic characteristics resemble those of Arabidopsis plants modified to over-express LCR. Taken together, the results demonstrate that MLPs are driven to degradation by LCR, and indicate that MLP gene family is target of miR394-LCR regulatory node, representing potential targets for directly post-translational regulation mediated by LCR F-Box. In addition, MLP28 family member is associated with the LCR regulation that is critical for normal Arabidopsis development.

Details

Title: Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis thaliana Identifies Major Latex Protein Family Members Critical for Normal Development
Authors: Celso Gaspar Litholdo, Jr (Author) - University of Sydney
Benjamin L Parker (Author) - University of Sydney
Andrew Eamens (Author) - University of Newcastle Australia
Martin R. Larsen (Author) - University of Southern Denmark
Stuart J. Cordwell (Author) - University of Sydney
Peter M. Waterhouse (Author) - University of Sydney
Publication details: Molecular & Cellular Proteomics, Vol.15(6), pp.2033-2047
Publisher: Elsevier Inc.
DOI: 10.1074/mcp.M115.053124
ISSN: 1535-9484
PMID: 27067051; PMC5083100
Organisation Unit: School of Health and Behavioural Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
Language: English
Record Identifier: 99656591302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemical Research Methods

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