Protein expression profiles of Chlamydia pneumoniae in models of persistence versus those of heat shock stress response

S Mukhopadhyay; R D Miller; E D Sullivan; C Theodoropoulos; S A Mathews; Peter Timms; J T Summersgill

doi:10.1128/IAI.02104-05

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Protein expression profiles of Chlamydia pneumoniae in models of persistence versus those of heat shock stress response

Journal article

Open access

Peer reviewed

Protein expression profiles of Chlamydia pneumoniae in models of persistence versus those of heat shock stress response

S Mukhopadhyay, R D Miller, E D Sullivan, C Theodoropoulos, S A Mathews, Peter Timms and J T Summersgill

Infection and immunity, Vol.74(7), pp.3853-3863

2006

DOI: https://doi.org/10.1128/IAI.02104-05

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Abstract

Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representing nine functional subgroups, from the gamma Interferon (IFN-?) treatment (primarily tryptophan limitation) and iron limitation (IL) models of persistence compared to those following heat shock (HS) at 42°C. Protein expression patterns of C. pneumoniae persistence indicates a strong stress component, as evidenced by the upregulation of proteins involved in protein folding, assembly, and modification. However, it is clearly more than just a stress response. In IFN persistence, but not IL or HS, amino acid and/or nucleotide biosynthesis proteins were found to be significantly upregulated. In contrast, proteins involved in the biosynthesis of cofactors, cellular processes, energy metabolism, transcription, and translation showed an increased in expression in only the IL model of persistence. These data represent the most extensive protein expression study of C. pneumoniae comparing the chlamydial heat shock stress response to two models of persistence and identifying the common and unique protein level responses during persistence. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Details

Title: Protein expression profiles of Chlamydia pneumoniae in models of persistence versus those of heat shock stress response
Authors: S Mukhopadhyay (Author) - University of Louisville School of Medicine, United States
R D Miller (Author) - University of Louisville School of Medicine, United States
E D Sullivan (Author) - University of Louisville School of Medicine, United States
C Theodoropoulos (Author) - Queensland University of Technology
S A Mathews (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
J T Summersgill (Author) - University of Louisville School of Medicine, United States
Publication details: Infection and immunity, Vol.74(7), pp.3853-3863
Publisher: American Society for Microbiology
Date published: 2006
DOI: 10.1128/IAI.02104-05
ISSN: 0019-9567
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449453702621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Infectious Diseases

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