Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus

C Rush; L Hafner; Peter Timms

doi:10.1007/s002530050969

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Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus

Journal article

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Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus

C Rush, L Hafner and Peter Timms

Applied Microbiology and Biotechnology, Vol.47(5), pp.537-542

1997

DOI: https://doi.org/10.1007/s002530050969

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Abstract

An expression system based on the Staphylococcus aureus protein A gene (spa) was developed to allow the production and export of proteins in Lactobacillus. Plasmid shuttle vectors were constructed that carried the eZZ gene, a synthetic gene based on the Protein A gene (spa) but lacking the carboxy-terminal membrane-anchoring region. A gene fusion was created between the eZZ gene and the VD4 region of a chlamydial major outer-membrane protein gene. Expression studies demonstrated the recognition of the spa regulatory signals by several Lactobacillus, with the recombinant protein being expressed (from 0.1 ?g of EZZVD4 fusion protein per ml in L. plantarum up to 10 ?g of EZZ protein per ml in L. fermentum) and exported (levels up to 20% in L. fermentum) in several Lactobacillus strains.

Details

Title: Protein A as a fusion partner for the expression of heterologous proteins in Lactobacillus
Authors: C Rush (Author) - Queensland University of Technology
L Hafner (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: Applied Microbiology and Biotechnology, Vol.47(5), pp.537-542
Publisher: Springer
Date published: 1997
DOI: 10.1007/s002530050969
ISSN: 0175-7598
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449890402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology

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