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Propagation Methods for Environmental Offset Planting of the Kogan Waxflower (Philotheca sporadica) (Rutaceae)
Journal article   Peer reviewed

Propagation Methods for Environmental Offset Planting of the Kogan Waxflower (Philotheca sporadica) (Rutaceae)

Brooke E Dwan and Stephen J Trueman
Journal of Environmental Science and Technology, Vol.7(6), pp.347-353
2014
DOI: https://doi.org/10.3923/jest.2014.347.353
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https://doi.org/10.3923/jest.2014.347.353View
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Abstract

conservation cuttings Eriostemon seed tissue culture translocation
The Kogan waxflower (Philotheca sporadica) is a threatened and geographically restricted plant species that is being impacted by gas pipeline construction. This study assessed seed, cutting and tissue culture methods for propagation of P. sporadica for an environmental offset program. Fresh seed could not be germinated under nursery conditions and less than 1.5% of cuttings produced roots. However, in vitro proliferation of shoots in tissue culture media containing 4.4 μM benzyladenine provided over 5,000 shoots from each initiated shoot tip in a 6 month period. Shoots were converted readily into plantlets using 314.9 μM indole-3-butyric acid for root induction and an in vitro soil-less system for transferring plantlets from the laboratory to the nursery. These results demonstrate that offset planting programs can be hampered by low amenability of plant species to propagation but that identifying a successful propagation method can lead to rapid gains in plant production.

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