Journal article
Progesterone Activates Multiple Innate Immune Pathways in Chlamydia trachomatis-Infected Endocervical Cells
American Journal of Reproductive Immunology, Vol.71(2), pp.165-177
2014
Abstract
Problem: Susceptibility to Chlamydia trachomatis infection is increased by oral contraceptives and modulated by sex hormones. We therefore sought to determine the effects of female sex hormones on the innate immune response to C. trachomatis infection. Method of study: ECC-1 endometrial cells, pre-treated with oestradiol or progesterone, were infected with C. trachomatis and the host transcriptome analysed by Illumina Sentrix HumanRef-8 microarray. Primary endocervical epithelial cells, prepared at either the proliferative or secretory phase of the menstrual cycle, were infected with C. trachomatis and cytokine gene expression determined by quantitative RT-PCR analysis. Results: Chlamydia trachomatis yield from progesterone-primed ECC-1 cells was significantly reduced compared with oestradiol-treated cells. Genes upregulated in progesterone-treated and Chlamydia-infected cells only included multiple CC and CXC chemokines, IL-17C, IL-29, IL-32, TNF-α, DEFB4B, LCN2, S100A7-9, ITGAM, NOD2, JAK1, IL-6ST, type I and II interferon receptors, numerous interferon-stimulated genes and STAT6. CXCL10, CXCL11, CX3CL1 and IL-17C, which were also upregulated in infected secretory-stage primary cells, and there was a trend towards higher levels of immune mediators in infected secretory-phase compared with proliferative-phase cells. Conclusion: Progesterone treatment primes multiple innate immune pathways in hormone-responsive epithelial cells that could potentially increase resistance to chlamydial infection.
Details
- Title
- Progesterone Activates Multiple Innate Immune Pathways in Chlamydia trachomatis-Infected Endocervical Cells
- Authors
- C Wan (Author) - Queensland University of TechnologyJ L Latter (Author) - University of NewcastleA Amirshahi (Author) - Queensland University of TechnologyI Symonds (Author) - University of NewcastleJ M Finnie (Author) - University of NewcastleN Bowden (Author) - University of NewcastleR J Scott (Author) - University of NewcastleK A Cunningham (Author) - Queensland University of TechnologyPeter Timms (Author) - Queensland University of TechnologyK W Beagley (Author) - Queensland University of Technology
- Publication details
- American Journal of Reproductive Immunology, Vol.71(2), pp.165-177
- Publisher
- Wiley-Blackwell Publishing Inc.
- Date published
- 2014
- DOI
- 10.1111/aji.12168
- ISSN
- 1046-7408
- Copyright note
- Copyright © 2014 The Author. This is the accepted version of the following article: Wan, C, Latter, J L, Amirshahi, A, Symonds, I, Finnie, J M, Bowden, N, Scott, R J, Cunningham, K A, Timms, Peter, Beagley, K W (2014) Progesterone Activates Multiple Innate Immune Pathways in Chlamydia trachomatis-Infected Endocervical Cells. American Journal of Reproductive Immunology, Vol. 71, No. 2, pp.165-177., which has been published in final form at http://dx.doi.org/10.1111/aji.12168
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450080402621
- Output Type
- Journal article
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- Domestic collaboration
- Web Of Science research areas
- Immunology
- Reproductive Biology
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