Journal article
Profiles of Entamoeba histolytica-specific immunoglobulins in human sera
Asian Pacific Journal of Tropical Medicine, Vol.5(3), pp.234-238
2012
Abstract
Objective: To determine the profiles of anti-Entamoeba histolytica (E. histolytica) IgA, IgG, and IgM in sera of diarrheic and non-diarrheic individuals and partially characterize target antigens. Methods: Serum samples from thirty diarrheic and thirty non-diarrheic individuals were subjected to IgA, IgG, and IgM profiling through enzyme-linked immunosorbent assay (ELISA), flow cytometry, and immunoblot. Results: ELISA titer results showed that both diarrheic and non-diarrheic individuals possess high levels of E. histolytica-specific IgG compared to IgA and IgM. Flow cytometry data showed that diarrheic serum samples had higher mean reaction percentages against E. histolytica cells compared to non-diarrheic samples. Immunoreactive E. histolytica proteins with molecular weights ranging between 7 kDa and 292 kDa were recognized by diarrheic serum IgG, and 170 kDa and 250 kDa by non-diarrheic serum IgG. Conclusions: Our findings suggest that serum anti-E. histolytica IgG, compared with serum anti-E. histolytica IgA and IgM responses, was generally high in both diarrheic and non-diarrheic sera, indicating a past exposure to the organism both in symptomatic patients as well as in asymptomatic carriers, respectively. In addition, serum IgG from diarrheic and non-diarrheic patients were able to detect immunogenic E. histolytica proteins. © 2012 Hainan Medical College.
Details
- Title
- Profiles of Entamoeba histolytica-specific immunoglobulins in human sera
- Authors
- W L Rivera (Author) - University of the Philippines, PhilippinesH J Santos (Author) - University of the Philippines, PhilippinesVanissa A Ong (Author) - University of the Philippines, PhilippinesL J G Murao (Author) - University of the Philippines, Philippines
- Publication details
- Asian Pacific Journal of Tropical Medicine, Vol.5(3), pp.234-238
- Publisher
- Elsevier Ltd.
- Date published
- 2012
- DOI
- 10.1016/S1995-7645(12)60031-5
- ISSN
- 1995-7645
- Copyright note
- Copyright © 2012 The Author. This work is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)
- Organisation Unit
- University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99451150202621
- Output Type
- Journal article
Metrics
166 File views/ downloads
790 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Web Of Science research areas
- Public, Environmental & Occupational Health
- Tropical Medicine
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites