Procoagulant role of microparticles in routine storage of packed red blood cells: potential risk for prothrombotic post-transfusion complications

H H Aung; J P Tung; Melinda M Dean; R L Flower; N M Pecheniuk

doi:10.1016/j.pathol.2016.10.001

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Procoagulant role of microparticles in routine storage of packed red blood cells: potential risk for prothrombotic post-transfusion complications

Journal article

Peer reviewed

Procoagulant role of microparticles in routine storage of packed red blood cells: potential risk for prothrombotic post-transfusion complications

H H Aung, J P Tung, Melinda M Dean, R L Flower and N M Pecheniuk

Pathology, Vol.49(1), pp.62-69

2017

DOI: https://doi.org/10.1016/j.pathol.2016.10.001

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Abstract

blood coagulation

blood transfusion

cell-derived microparticles

erythrocyte transfusion

phospholipids

During routine storage, packed red blood cells (PRBC) undergo biochemical and morphological changes including loss of red blood cell (RBC) membrane asymmetry and release of microparticles (MPs) bearing phosphatidylserine (PS), a procoagulant phospholipid. This study investigated the association between PRBC storage duration, MP profile and procoagulant activity. Leukodepleted PRBC-supernatant (PRBC-SN; n = 13) was prepared at weekly intervals throughout storage. Phospholipid-dependent procoagulant activity, assessed using a factor X-activated clotting time (XACT) assay, decreased throughout storage (p < 0.0001), corresponding with increased procoagulant phospholipid content. As determined by flow cytometry, total numbers of MPs and of PS-bearing MPs increased by Day 28 of storage (p < 0.01 and p < 0.05, respectively, versus D1), and these MPs were predominantly RBC-derived (CD235+). Depletion of MPs from stored (Day 42) PRBC-SN using 0.22 μm filters reduced the number of PS-bearing MPs (p < 0.01) but did not increase XACT clotting times. Furthermore, the reduction in procoagulant activity when lactadherin was used to block PS was not altered pre- or post-filtration of PRBC-SN. In conclusion, routine PRBC storage was associated with accumulation of MPs (particularly RBC-derived PS-bearing MPs) and of procoagulant phospholipids; however, depletion of PS-bearing MPs by 0.22 μm filtration did not reduce phospholipid-dependent procoagulant activity.

Details

Title: Procoagulant role of microparticles in routine storage of packed red blood cells: potential risk for prothrombotic post-transfusion complications
Authors: H H Aung (Author) - Queensland University of Technology
J P Tung (Author) - Queensland University of Technology
Melinda M Dean (Author) - Queensland University of Technology
R L Flower (Author) - Queensland University of Technology
N M Pecheniuk (Author) - Queensland University of Technology
Publication details: Pathology, Vol.49(1), pp.62-69
Publisher: Elsevier Ltd.
Date published: 2017
DOI: 10.1016/j.pathol.2016.10.001
ISSN: 0031-3025
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99450790902621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Pathology

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