Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR

Warish Ahmed; S Sawant; F Huygens; A Goonetilleke; Ted Gardner

doi:10.1016/j.watres.2009.03.041

Back

Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR

Journal article

Open access

Peer reviewed

Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR

Warish Ahmed, S Sawant, F Huygens, A Goonetilleke and Ted Gardner

Water Research, Vol.43(19), pp.4918-4928

2009

DOI: https://doi.org/10.1016/j.watres.2009.03.041

Files and links (2)

pdf

PDF - Author's Accepted Version (Open Access)287.74 kBDownload View

Accepted VersionPDF - Author Accepted Version (Open Access)CC BY-NC-ND V4.0, Open Access

url

https://doi.org/10.1016/j.watres.2009.03.041View

Published Version

Abstract

surface water

fecal pollution

zoonotic pathogens

fecal indicators

health risk

The prevalence and concentrations of Campylobacter jejuni, Salmonella spp. and enterohaemorrhagic Escherichia coli (EHEC) were investigated in surface waters in Brisbane, Australia using quantitative PCR (qPCR) based methodologies. Water samples were collected from Brisbane City Botanic Gardens (CBG) Pond, and two urban tidal creeks (i.e., Oxley Creek and Blunder Creek). Of the 32 water samples collected, 8 (25%), 1 (3%), 9 (28%), 14 (44%), and 15 (47%) were positive for C. jejuni mapA, Salmonella invA, EHEC O157 LPS, EHEC VT1, and EHEC VT2 genes, respectively. The presence/absence of the potential pathogens did not correlate with either E. coli or enterococci concentrations as determined by binary logistic regression. In conclusion, the high prevalence, and concentrations of potential zoonotic pathogens along with the concentrations of one or more fecal indicators in surface water samples indicate a poor level of microbial quality of surface water, and could represent a significant health risk to users. The results from the current study would provide valuable information to the water quality managers in terms of minimizing the risk from pathogens in surface waters. © 2009.

Details

Title: Prevalence and occurrence of zoonotic bacterial pathogens in surface waters determined by quantitative PCR
Authors: Warish Ahmed (Author) - Department of Natural Resources and Water
S Sawant (Author) - Queensland University of Technology
F Huygens (Author) - Queensland University of Technology
A Goonetilleke (Author) - Queensland University of Technology
Ted Gardner (Author) - Queensland University of Technology
Publication details: Water Research, Vol.43(19), pp.4918-4928
Publisher: IWA Publishing
Date published: 2009
DOI: 10.1016/j.watres.2009.03.041
ISSN: 0043-1354
Copyright note: Copyright © 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451148802621
Output Type: Journal article

Metrics

107 File views/ downloads

481 Record Views

83 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Web Of Science research areas: Engineering, Environmental; Environmental Sciences; Water Resources

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites