Preservation of encapsulated shoot tips and nodes of the tropical hardwoods Corymbia torelliana × C. citriodora and Khaya senegalensis

Cao Dinh Hung; Stephen J Trueman

doi:10.1007/s11240-011-0099-0

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Preservation of encapsulated shoot tips and nodes of the tropical hardwoods Corymbia torelliana × C. citriodora and Khaya senegalensis

Journal article

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Peer reviewed

Preservation of encapsulated shoot tips and nodes of the tropical hardwoods Corymbia torelliana × C. citriodora and Khaya senegalensis

Cao Dinh Hung and Stephen J Trueman

Plant Cell, Tissue and Organ Culture, Vol.109(2), pp.341-352

2012

DOI: https://doi.org/10.1007/s11240-011-0099-0

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Abstract

Eucalyptus

mahogany

Meliaceae

Myrtaceae

synthetic seed

tissue culture

Alginate encapsulation is a simple and cost-effective technique to preserve plant germplasm but there are only a few reports available on preservation of encapsulated explants of two highly valuable groups of tropical trees, the eucalypts (Myrtaceae) and mahoganies (Meliaceae). This study investigated alginate encapsulation for preservation of the eucalypt hybrid, Corymbia torelliana × C. citriodora, and the African mahogany, Khaya senegalensis. We assessed shoot regrowth of encapsulated shoot tips and nodes after storage for 0, 3, 6 and 12 months on media varying in sucrose and nutrient content, under storage conditions of 14°C and zero-irradiance. Encapsulated explants of both trees were preserved most effectively on high-nutrient (half-strength Murashige and Skoog) medium containing 1% sucrose, which provided very high frequencies of shoot regrowth (92–100% for Corymbia and 71–98% for Khaya) and excellent shoot development after 12 months’ storage. This technique provides an extremely efficient means for storage and exchange of eucalypts and mahoganies, ideally suited for incorporation into plant breeding and germplasm conservation programs.

Details

Title: Preservation of encapsulated shoot tips and nodes of the tropical hardwoods Corymbia torelliana × C. citriodora and Khaya senegalensis
Authors: Cao Dinh Hung (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Stephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Plant Cell, Tissue and Organ Culture, Vol.109(2), pp.341-352
Publisher: Springer Netherlands
Date published: 2012
DOI: 10.1007/s11240-011-0099-0
ISSN: 0167-6857
Copyright note: Copyright © 2012 Springer Netherlands. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final definitive publication is available at www.springerlink.com
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99449592802621
Output Type: Journal article

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