Pre-storage leucoreduction of platelet concentrates reduced biological response modifier concentrations and TRALI incidence in an in vivo sheep model

John-Paul Tung; Gabriela Simonova; Elise Hewlett; Sanne Engkilde Pedersen; Arlanna Esguerra Lallen; Fahad Alkhureeb; Rebecca Wellburn; Sara Chiaretti; Fergal T Temple; Thy Duong; Michelle Cinel; Corey Giles; Peter J Meikle; John F Fraser

doi:10.1038/s41598-025-26159-x

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Pre-storage leucoreduction of platelet concentrates reduced biological response modifier concentrations and TRALI incidence in an in vivo sheep model

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Pre-storage leucoreduction of platelet concentrates reduced biological response modifier concentrations and TRALI incidence in an in vivo sheep model

John-Paul Tung, Gabriela Simonova, Elise Hewlett, Sanne Engkilde Pedersen, Arlanna Esguerra Lallen, Fahad Alkhureeb, Rebecca Wellburn, Sara Chiaretti, Fergal T Temple, Thy Duong, …

Scientific Reports, Vol.15, pp.1-16

2025

DOI: https://doi.org/10.1038/s41598-025-26159-x

PMCID: PMC12657934

PMID: 41298662

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Abstract

transfusion-related lung injury

TRALI

Leucoredection

sheep model

Transfusion-related acute lung injury (TRALI) is a potentially fatal transfusion reaction. It can be caused by the transfusion of proteins, lipids, and extracellular vesicles that accumulate in blood components during routine storage and are collectively referred to as biological response modifiers (BRMs). It has been suggested that leucoreduction might reduce TRALI risk by limiting the accumulation of leucocyte-derived BRMs; however, there are conflicting data from haemovigilance reports and animal models regarding this. We harvested supernatant (SN) from leucoreduced buffy coat pooled platelet concentrates (PCs) at days 2, 5, or 7 (n = 25 each). The SNs from each time-point were pooled, aliquoted, and frozen. Similar SN pools collected from non-leucoreduced PCs in a previous study were also used. The PC SN pools underwent analyses for protein, extracellular vesicle and lipid BRMs. The leucoreduced PC SN pools were also transfused to lipopolysaccharide-treated sheep for TRALI assessment (n = 7 per group), with a further four sheep receiving saline infusion as a control. Leucoreduction limited storage-related accumulation of several potential BRMs. This was associated with a lower incidence of TRALI compared to our previous study using non-leucoreduced PC SN. Sheep who developed TRALI had lower plasma levels of interleukin-10, providing further evidence that it may play a protective role against TRALI development.

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Title: Pre-storage leucoreduction of platelet concentrates reduced biological response modifier concentrations and TRALI incidence in an in vivo sheep model
Authors: John-Paul Tung (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Health and Behavioural Sciences - Legacy
Gabriela Simonova - Australian Red Cross Lifeblood
Elise Hewlett - Australian Red Cross Lifeblood
Sanne Engkilde Pedersen - Critical Care Research Group, The Prince Charles Hospital, Brisbane, QLD, Australia
Arlanna Esguerra Lallen - Australian Red Cross Lifeblood
Fahad Alkhureeb - Australian Red Cross Lifeblood
Rebecca Wellburn - Australian Red Cross Lifeblood
Sara Chiaretti - Australian Red Cross Lifeblood
Fergal T Temple - Australian Red Cross Lifeblood
Thy Duong - Baker Heart and Diabetes Institute
Michelle Cinel - Baker Heart and Diabetes Institute
Corey Giles - Baker Heart and Diabetes Institute
Peter J Meikle - Baker Heart and Diabetes Institute
John F Fraser - The University of Queensland
Publication details: Scientific Reports, Vol.15, pp.1-16
Publisher: Nature Publishing Group
Date published: 2025
DOI: 10.1038/s41598-025-26159-x
ISSN: 2045-2322
PMID: 41298662; PMC12657934
Copyright note: This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.
Data Availability: The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Grants: Integrating lipidomic, metabolomic and genomic studies to define causal metabolic pathways in cardiometabolic disease: Delivering precision medicine solutions for Australia., 2009965, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Grant note: This work was supported by internal research project funding from Australian Red Cross Lifeblood.
Organisation Unit: School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 991188344302621
Output Type: Journal article

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