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Journal article
Potent Ovarian Development as Being Stimulated by Cocktail Hormone in the Female Scylla olivacea
Frontiers of Marine Science, Vol.10, pp.1-14
2023
Abstract
The mud crab Scylla olivacea is widely cultured for its economic value, but reproduction issues limit its production. Vitellogenesis-inhibiting hormone (VIH), serotonin (5-HT), and gonadotropin-releasing hormone (GnRH) are important neurohormones that control reproduction in crustaceans. Mimicking crab hormone stimulation during reproduction has scarcely been reported. Comparison of the single hormone and multiple hormone approaches to crab hormonal control in S. olivacea is limited. In situ hybridization showed that injection of dsRNA-VIH could abolish its gene expression in neuronal clusters of female S. olivacea eyestalks, potentially reducing its inhibitory effects on ovarian maturation. This was confirmed by assessing the ovarian gonadosomatic index (GSI), hemolymph vitellogenin (Vg), an indicator of vitellogenesis, and gonad histology using dsRNA-VIH and 5-HT/GnRH combinations. Based on our findings, we demonstrated that administration of dsRNA-VIH significantly increased the gonadosomatic index (GSI) on days 14 and 28 post-treatment. The combination cocktail, however, consisting of 5-HT + GnRH + dsRNA-VIH on days 14 and 28, and GnRH + dsRNA-VIH on day 28, was the most efficacious in increasing GSI and enhancing crab ovarian maturation. Upregulation of hemolymph Vg levels was observed solely on the 28th day following treatment with dsRNA-VIH, 5-HT + GnRH + dsRNA-VIH, and GnRH + dsRNA-VIH. Differential gene expression analysis using quantitative RNA-sequencing of the neural tissues (brain and ventral nerve cord), revealed a significant upregulation of certain receptors (5-HTR, GnRHR, LHR, and FSHR), neuropeptides (sNPF, NPF1, NPF2, SIFamide, AKH/Crz, CHH, and RPCH), downstream reproductive-related genes (FAMeT, ESULT, progesterone-like protein), and prostanoid-related genes (phospholipase A and C, COX, Thromboxane A synthase, prostaglandin D, E, and F synthases) following treatment, particularly dsRNA-VIH + GnRH and/or 5-HT-injected individuals. Upregulation of prostaglandin E synthase and estrogen sulfotransferase genes was confirmed by real-time PCR. Since the construction and propagation of dsRNA-VIH is costly, its lower dose application supplemented with synthetic GnRH and/or 5-HT may be an alternative approach to ensure that female S. olivacea attain sufficient reproductive fecundity in aquaculture. Furthermore, we propose that the administration of multiple hormones in crabs may better emulate the physiological conditions of crustaceans in their natural habitat.
Details
- Title
- Potent Ovarian Development as Being Stimulated by Cocktail Hormone in the Female Scylla olivacea
- Authors
- Jirawat Saetan (Author) - Prince of Songkla UniversitySupawadee Duangprom (Author) - Thammasat UniversitySineenart Songkoomkrong (Author) - Thammasat UniversityPrateep Amonruttanapun (Author) - Thammasat UniversityTeva Phanaksri (Author) - Thammasat UniversityPiyaporn Surinlert (Author) - Thammasat UniversityChompunut Samhuay (Author) - Ministry of Agriculture and CooperativesMontakan Tamtin (Author) - Ministry of Agriculture and CooperativesSaowaros Suwansa-ard (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringScott Cummins (Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and EngineeringPrasert Sobhon (Author) - Mahidol UniversityNapamanee Kornthong (Corresponding Author) - Thammasat University
- Publication details
- Frontiers of Marine Science, Vol.10, pp.1-14
- Publisher
- Frontiers Research Foundation
- Date published
- 2023
- DOI
- 10.3389/fmars.2023.1286789
- ISSN
- 2296-7745
- Copyright note
- © 2023 Saetan, Duangprom, Songkoomkrong, Amonruttanapun, Phanaksri, Surinlert, Samhuay, Tamtin, Suwansa-Ard, Cummins, Sobhon and Kornthong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Grant note
- This work was supported by Thammasat University Research Unit in Biotechnology and its application of aquatic animals. The authors also gratefully acknowledge the financial support provided by Thailand Science Research and Innovation Fundamental Fund (TUFF 60/2565).
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99982897802621
- Output Type
- Journal article
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