Population structure and uropathogenic virulence associated genes of faecal Escherichia coli from healthy young and elderly adults

Tara L Vollmerhausen; Nubia Ramos; Aycan Gundogdu; Wayne A Robinson; A Brauner; Mohammad Katouli

doi:10.1099/jmm.0.027037-0

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Population structure and uropathogenic virulence associated genes of faecal Escherichia coli from healthy young and elderly adults

Journal article

Open access

Peer reviewed

Population structure and uropathogenic virulence associated genes of faecal Escherichia coli from healthy young and elderly adults

Tara L Vollmerhausen, Nubia Ramos, Aycan Gundogdu, Wayne A Robinson, A Brauner and Mohammad Katouli

Journal of Medical Microbiology, Vol.60(5), pp.574-581

2011

DOI: https://doi.org/10.1099/jmm.0.027037-0

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Abstract

health

Escherichia coli

microbiology

We investigated the population structures of faecal Escherichia coli in 30 healthy young adults (13 males and 17 females) aged between 20 and 45 years and 29 elderly adults (14 females and 15 males) aged between 65 and 77 years. In all, 1566 strains were typed with the PhPlate system and grouped into biochemical phenotypes (BPTs). Strains with shared BPTs were further typed using randomly amplified polymorphic DNA analysis. Forty-four per cent of the strains were shared between two or more age and gender groups. Elders had a significantly higher (P,0.001) number of BPTs (mean±standard error 3.3±0.27) than younger groups (1.82±0.27). Phylogenetic affiliation and virulence-associated genes (VAGs) of the strains showed that more than 80% of the strains belonging to dominant types belonged to phylogroups B2 and D. Amongst dominant BPTs, phylogenetic group A was significantly associated with females (P,0.0001), and elders were more likely to carry group D (P,0.0124). Elderly males had a higher prevalence of VAGs han young males (P,0.0001) and young females (P,0.0005). We conclude that there is a lower prevalence of E. coli with uropathogenic properties in healthy young adults than in elders.

Details

Title: Population structure and uropathogenic virulence associated genes of faecal Escherichia coli from healthy young and elderly adults
Authors: Tara L Vollmerhausen (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Nubia Ramos (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Aycan Gundogdu (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Wayne A Robinson (Author) - Charles Sturt University
A Brauner (Author) - Karolinska University Hospital, Sweden
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Journal of Medical Microbiology, Vol.60(5), pp.574-581
Publisher: Society for General Microbiology
Date published: 2011
DOI: 10.1099/jmm.0.027037-0
ISSN: 0022-2615
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449681402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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