Journal article
Polymeric microneedle patches for rapid DNA extraction and molecular diagnosis of foliar pathogens of mung bean
Talanta, Vol.311, pp.1-12
2027
PMID: 42456256
Abstract
Global food security is threatened by crop losses due to plant diseases. Early detection of pathogens is essential for implementing effective management practices to mitigate the negative impacts of disease. On-site molecular diagnostic techniques have significantly advanced for rapid and accurate identification of pathogens, but DNA extraction remains a labour-intensive and time-consuming bottleneck. This study demonstrates the ability of polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) microneedle patches to extract detectable amounts of bacterial and plant DNA from diseased leaves within one minute. This included DNA of a gram-negative Pseudomonas savastanoi pv. phaseolicola (Psp) and a gram-positive Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) bacteria, as well as mung bean (Vigna radiata). These bacteria cause halo blight and tan spot of mung bean, respectively. The DNA extracted using microneedle patches was of adequate quality and quantity to identify pathogens using real-time quantitative and droplet digital PCR (ddPCR). For leaves expressing halo blight symptoms, Psp DNA was detected in all DNA templates from microneedle (n = 5) and conventional extractions (n = 5). Similarly, in a time course study, Psp inoculated samples at day 6 showed the same detection in templates collected with microneedles and conventionally extracted samples. Detection of Cff DNA was less frequent, suggesting an impact of the sampling or inoculation technique. This study supports ongoing development of microneedle-based extraction as a simple and cost-effective method that could be used to support diagnosis of plant diseases, facilitate rapid decision-making for disease management, enhance biosecurity responses and area-freedom datasets, and prevent crop losses.
Details
- Title
- Polymeric microneedle patches for rapid DNA extraction and molecular diagnosis of foliar pathogens of mung bean
- Authors
- Asim Mushtaq - University of Southern QueenslandBarsha Poudel - University of Southern QueenslandAlina Karki - University of Southern QueenslandPouria Azarikhah - University of Southern QueenslandAhmed Saad - University of Southern QueenslandAdrian Dinsdale - Department of Agriculture, Fisheries and ForestrySonia Fiorito - Department of Agriculture, Fisheries and ForestryNoel L. Knight - University of Southern QueenslandZahra Faraji Rad (Corresponding Author) - University of the Sunshine Coast
- Publication details
- Talanta, Vol.311, pp.1-12
- Publisher
- Elsevier BV
- Date published
- 2027
- DOI
- 10.1016/j.talanta.2026.130267
- ISSN
- 1873-3573
- PMID
- 42456256
- Copyright note
- © 2026 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ ).
- Data Availability
- Data will be made available on request.
- Grant note
- This research was funded by the Australian Government Department of Agriculture, Fisheries and Forestry's Biosecurity Innovation Project (Project D157) and the Broadacre Cropping Initiative, a joint investment of the University of Southern Queensland and the Queensland Government Department of Primary Industries.
- Organisation Unit
- School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 991246281102621
- Output Type
- Journal article
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