Poly(amino acids) at Si-oxide interfaces - Bio-colloidal interactions, adhesion and 'conformation'

Gregory S Watson; Jolanta A Blach; C Cahill; D V Nicolau; D K Pham; J Wright; S Myhra

doi:10.1007/s00396-003-0914-1

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Poly(amino acids) at Si-oxide interfaces - Bio-colloidal interactions, adhesion and 'conformation'

Journal article

Peer reviewed

Poly(amino acids) at Si-oxide interfaces - Bio-colloidal interactions, adhesion and 'conformation'

Gregory S Watson, Jolanta A Blach, C Cahill, D V Nicolau, D K Pham, J Wright and S Myhra

Colloid and Polymer Science, Vol.282(1), pp.56-63

2003

DOI: https://doi.org/10.1007/s00396-003-0914-1

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Abstract

double layer interactions

poly(amino acids)

colloidal probe microscopy

DLVO model

Biopolymers in a confined solution environment will be subject to long-range electrostatic and short-range van der Waals interactions. The interactions between two model poly(amino acids), poly-L-lysine and poly-L-glutamic acid, in solution with a Si-oxide surface have been investigated. The model amino acids were adsorbed, or covalently coupled, to colloidal probes consisting of a micro-sphere attached to a force-sensing lever. The methodology was based on sensing interaction between the probe and a flat surface through carrying out force versus distance analysis with a scanning force microscope. The framework of the conventional DLVO theory was used to arrive at a qualitative description. The outcomes illustrate both repulsive and attractive long-range interactions that will hinder, or promote, transport of colloidal biospecies into the region of attractive short-range interactions at the physical interface. Large 'snap-on' distances were observed for some systems and were ascribed to compression of the 'soft' functionalised layers. Those observations and measurements of adhesion provided insights into conformation of the adsorbed species and strength of attachment. The results are relevant to design and functionality of Si-based microfluidics technology. © Springer-Verlag 2003.

Details

Title: Poly(amino acids) at Si-oxide interfaces - Bio-colloidal interactions, adhesion and 'conformation'
Authors: Gregory S Watson (Author) - Griffith University
Jolanta A Blach (Author) - Griffith University
C Cahill (Author) - Griffith University
D V Nicolau (Author) - Swinburne University of Technology
D K Pham (Author) - Swinburne University of Technology
J Wright (Author) - Swinburne University of Technology
S Myhra (Author) - Griffith University
Publication details: Colloid and Polymer Science, Vol.282(1), pp.56-63
Publisher: Springer
Date published: 2003
DOI: 10.1007/s00396-003-0914-1
ISSN: 0303-402X
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering
Language: English
Record Identifier: 99449185502621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Chemistry, Physical; Polymer Science