Journal article
Platelet-activating factor and serum components from oestrous mice co-operate to mimic the activity of early pregnancy factor in the rosette inhibition assay
Journal of Reproduction & Fertility, Vol.88(2), pp.447-457
1990
PMID: 2325013
Abstract
When male mouse spleen cells were incubated with a combination of platelet activating factor (PAF, 1-0-alkyl-2-acetyl-sn-glycero-3-phosphocholine) and sera from female mice in oestrus, the cells displayed a markedly increased rosette inhibition titre (RIT) when subsequently tested in the rosette inhibition assay. Neither PAF nor oestrous mouse sera alone could induce this effect, the combined action was required. Lyso-PAF could not substitute for the PAF, nor could male mouse sera nor the sera from females in dioestrus or metoestrus substitute for the oestrous mouse serum requirement. Pro-oestrous mouse sera could replace oestrous mouse sera but were less effective in their dose–responses. Studies on the mechanism of action of the PAF and oestrous mouse serum components suggested that the PAF stimulated the production and release of soluble factors (termed S2 factors) which by themselves could induce increased RIT values when applied to fresh spleen cells. The PAF-stimulated cell populations were rendered refractory to the action of these S2 factors and did not display increased RIT values, unless oestrous mouse serum was added. This serum acted to reverse the refractory state, allowing the S2 factors to exert their effect, and so cells treated with PAF and oestrous mouse serum displayed increased RIT values.
Details
- Title
- Platelet-activating factor and serum components from oestrous mice co-operate to mimic the activity of early pregnancy factor in the rosette inhibition assay
- Authors
- C Orozco (Author) - Griffith UniversityI Cock (Author) - Griffith UniversityA. V Perkins (Author) - Griffith UniversityF. M Clarke (Author) - Griffith University
- Publication details
- Journal of Reproduction & Fertility, Vol.88(2), pp.447-457
- Publisher
- Society for Reproduction and Fertility
- DOI
- 10.1530/jrf.0.0880447
- ISSN
- 1471-7899
- PMID
- 2325013
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; School of Health
- Language
- English
- Record Identifier
- 99685194402621
- Output Type
- Journal article
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- Reproductive Biology
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