Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis

Erin P Price; Derek S Sarovich; Jessica R Webb; Carina M Hall; Sierra A Jaramillo; Jason W Sahl; Mirjam Kaestli; Mark Mayo; Glenda Harrington; Anthony L Baker; Lindsay C Sidak-Loftis; Erik W Settles; Madeline Lummis; James M Schupp; John D Gillece; Apichai Tuanyok; Jeffrey Warner; Joseph D Busch; Paul Keim; Bart Currie; David M Wagner

doi:10.1371/journal.pntd.0005928

Back

Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis

Journal article

Open access

Peer reviewed

Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis

Erin P Price, Derek S Sarovich, Jessica R Webb, Carina M Hall, Sierra A Jaramillo, Jason W Sahl, Mirjam Kaestli, Mark Mayo, Glenda Harrington, Anthony L Baker, …

PLoS Neglected Tropical Diseases, Vol.11(9), e0005928

2017

DOI: https://doi.org/10.1371/journal.pntd.0005928

Files and links (2)

pdf

PDF - Published Version (Open Access)6.09 MBDownload View

Published VersionPDF - Published Version (Open Access)CC BY V4.0, Open Access

url

https://doi.org/10.1371/journal.pntd.0005928View

Published Version

Abstract

The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudomallei. Unlike most other Burkholderia cepacia complex (Bcc) species, B. ubonensis is considered non-pathogenic, although its virulence potential has not been tested. Antibiotic resistance in B. ubonensis, particularly towards drugs used to treat the most severe B. pseudomallei infections, has also been poorly characterised. This study examined the population biology of B. ubonensis, and includes the first reported isolates from the Caribbean. Phylogenomic analysis of 264 B. ubonensis genomes identified distinct clades that corresponded with geographic origin, similar to B. pseudomallei. A small proportion (4%) of strains lacked the 920kb chromosome III replicon, with discordance of presence/absence amongst genetically highly related strains, demonstrating that the third chromosome of B. ubonensis, like other Bcc species, probably encodes for a nonessential pC3 megaplasmid. Multilocus sequence typing using the B. pseudomallei scheme revealed that one-third of strains lack the "housekeeping" narK locus. In comparison, all strains could be genotyped using the Bcc scheme. Several strains possessed high-level meropenem resistance (≥32 μg/mL), a concern due to potential transmission of this phenotype to B. pseudomallei. In silico analysis uncovered a high degree of heterogeneity among the lipopolysaccharide O-antigen cluster loci, with at least 35 different variants identified. Finally, we show that Asian B. ubonensis isolate RF23-BP41 is avirulent in the BALB/c mouse model via a subcutaneous route of infection. Our results provide several new insights into the biology of this understudied species.

Details

Title: Phylogeographic, genomic, and meropenem susceptibility analysis of Burkholderia ubonensis
Authors: Erin P Price (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Derek S Sarovich (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Jessica R Webb (Author) - Menzies School of Health Research
Carina M Hall (Author) - Northern Arizona University, United States
Sierra A Jaramillo (Author) - Northern Arizona University, United States
Jason W Sahl (Author) - Northern Arizona University, United States
Mirjam Kaestli (Author) - Menzies School of Health Research
Mark Mayo (Author) - Menzies School of Health Research
Glenda Harrington (Author) - Menzies School of Health Research
Anthony L Baker (Author) - James Cook University
Lindsay C Sidak-Loftis (Author) - Northern Arizona University, United States
Erik W Settles (Author) - Northern Arizona University, United States
Madeline Lummis (Author) - Translational Genomics Research Institute
James M Schupp (Author) - Translational Genomics Research Institute, United States
John D Gillece (Author) - Translational Genomics Research Institute, United States
Apichai Tuanyok (Author) - Northern Arizona University, United States
Jeffrey Warner (Author) - James Cook University
Joseph D Busch (Author) - Northern Arizona University, United States
Paul Keim (Author) - Northern Arizona University, United States
Bart Currie (Author) - Menzies School of Health Research
David M Wagner (Author) - Northern Arizona University, United States
Publication details: PLoS Neglected Tropical Diseases, Vol.11(9), e0005928; 18
Publisher: Public Library of Science
Date published: 2017
DOI: 10.1371/journal.pntd.0005928
ISSN: 1935-2727
Copyright note: Copyright © 2017 Price et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450917302621
Output Type: Journal article

Metrics

165 File views/ downloads

313 Record Views

18 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Parasitology; Tropical Medicine

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites