Phylogenetic analysis of human Chlamydia pneumoniae strains reveals a distinct Australian indigenous clade that predates European exploration of the continent

Eileen Roulis; Nathan Bachmann; Michael Humphrys; Garry Myers; Wilhelmina Huston; Adam Polkinghorne; Peter Timms

doi:10.1186/s12864-015-2281-y

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Phylogenetic analysis of human Chlamydia pneumoniae strains reveals a distinct Australian indigenous clade that predates European exploration of the continent

Journal article

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Phylogenetic analysis of human Chlamydia pneumoniae strains reveals a distinct Australian indigenous clade that predates European exploration of the continent

Eileen Roulis, Nathan Bachmann, Michael Humphrys, Garry Myers, Wilhelmina Huston, Adam Polkinghorne and Peter Timms

BMC Genomics, Vol.16, pp.1-15

2015

DOI: https://doi.org/10.1186/s12864-015-2281-y

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Abstract

Chlamydia pneumoniae

whole genome sequencing

phylogenomics

polymorphisms

inclusion proteins

polymorphic membrane protein

inosine-monophosphate dehydrogenase

recombination

infection

Background: The obligate intracellular bacterium Chlamydia pneumoniae is a common respiratory pathogen, which has been found in a range of hosts including humans, marsupials and amphibians. Whole genome comparisons of human C. pneumoniae have previously highlighted a highly conserved nucleotide sequence, with minor but key polymorphisms and additional coding capacity when human and animal strains are compared. Results: In this study, we sequenced three Australian human C. pneumoniae strains, two of which were isolated from patients in remote indigenous communities, and compared them to all available C. pneumoniae genomes. Our study demonstrated a phylogenetically distinct human C. pneumoniae clade containing the two indigenous Australian strains, with estimates that the most recent common ancestor of these strains predates the arrival of European settlers to Australia. We describe several polymorphisms characteristic to these strains, some of which are similar in sequence to animal C. pneumoniae strains, as well as evidence to suggest that several recombination events have shaped these distinct strains. Conclusions: Our study reveals a greater sequence diversity amongst both human and animal C. pneumoniae strains, and suggests that a wider range of strains may be circulating in the human population than current sampling indicates.

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Title: Phylogenetic analysis of human Chlamydia pneumoniae strains reveals a distinct Australian indigenous clade that predates European exploration of the continent
Authors: Eileen Roulis (Author) - Queensland University of Technology
Nathan Bachmann (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Michael Humphrys (Author) - University of Maryland, United States
Garry Myers (Author) - University of Technology, Sydney
Wilhelmina Huston (Author) - Queensland University of Technology
Adam Polkinghorne (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Peter Timms (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: BMC Genomics, Vol.16, pp.1-15
Publisher: BioMed Central Ltd.
Date published: 2015
DOI: 10.1186/s12864-015-2281-y
ISSN: 1471-2164
Copyright note: Copyright © 2015 Roulis et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Grants: Specificity of Chlamydia pneumoniae for humans: analysis of previous cross-host transmission events and the role of tryptophan availability in host specificity, 1004666, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Organisation Unit: Centre for Bioinnovation
Language: English
Record Identifier: 99449109702621
Output Type: Journal article

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