Phenotypic Diversity of Multiple Antibiotic Resistant Enterococci with Emphasis on Enterococcus gallinarum Carrying vanA and vanB Genes

B Shaghaghi; M Talebi; Mohammad Katouli; R Mollby; I Kuhn; M R Pourshafie

doi:10.1007/s11270-007-9481-2

Back

Phenotypic Diversity of Multiple Antibiotic Resistant Enterococci with Emphasis on Enterococcus gallinarum Carrying vanA and vanB Genes

Journal article

Peer reviewed

Phenotypic Diversity of Multiple Antibiotic Resistant Enterococci with Emphasis on Enterococcus gallinarum Carrying vanA and vanB Genes

B Shaghaghi, M Talebi, Mohammad Katouli, R Mollby, I Kuhn and M R Pourshafie

Water, Air and Soil Pollution, Vol.186(1-4), pp.255-261

2007

DOI: https://doi.org/10.1007/s11270-007-9481-2

Files and links (1)

url

https://doi.org/10.1007/s11270-007-9481-2View

Published Version

Abstract

antibiotic resistance

wastewater

Enterococcus gallinarum

biochemical fingerprinting

The prevalence and diversity of antibiotic resistant enterococci populations in samples collected four times from urban sewage treatment plant in Tehran, Iran between June 2005 and July 2006 were studied. Filtered samples were grown on mEnterococci medium containing 4 μg/ml vancomycin after which the enterococci isolates were identified to the species level. All strains were then tested for their resistance against nine antibiotics. Of the 131 isolates, 98 (75%) isolates were identified as Enterococcus gallinarum, followed by 24 (18%) and 9 (7%) for E. faecium and E. casseliflavus, respectively. All E. gallinarum isolates carried vanC1 gene with 64 (65%) and 14 (14%) isolates concomitantly harboured either vanA or vanB gene, respectively. Some E. casseliflavus concomitantly harboured vanA and vanC2 or vanB and vanC2. Typing the total enterococci isolates with a high resolution biochemical fingerprinting method showed a high diversity (D i = 0.91). We have shown by biochemical fingerprinting the presence of highly diverse glycopeptide resistant E. gallinarum and E. casseliflavus that have captured vanA and vanB genetic determinants under natural conditions. To our knowledge this is the first report in this geographical region showing high frequency antibiotic resistant enterococcal populations in particular E. gallinarum carrying assorted vancomycin resistance genes.

Details

Title: Phenotypic Diversity of Multiple Antibiotic Resistant Enterococci with Emphasis on Enterococcus gallinarum Carrying vanA and vanB Genes
Authors: B Shaghaghi (Author) - Pasteur Institute of Iran, Iran
M Talebi (Author) - Pasteur Institute of Iran, Iran
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
R Mollby (Author) - Karolinska Institute, Sweden
I Kuhn (Author) - Karolinska Institute, Sweden
M R Pourshafie (Author) - Pasteur Institute of Iran, Iran
Publication details: Water, Air and Soil Pollution, Vol.186(1-4), pp.255-261
Publisher: Springer Netherlands
Date published: 2007
DOI: 10.1007/s11270-007-9481-2
ISSN: 0049-6979
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99449360802621
Output Type: Journal article

Metrics

1 File views/ downloads

509 Record Views

6 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites