Journal article
Phasing the meridional diffraction pattern of type I collagen using isomorphous derivatives
Journal of Molecular Biology, Vol.205(4), pp.685-694
1989
Abstract
The meridional X-ray diffraction pattern of wet rat tail tendon contains information about the one-dimensional structure, or axial projection of electron density distribution of the type I collagen fibril. Using synchrotron radiation we have determined the intensities of the first 50 meridional X-ray diffraction reflections. The approach of isomorphous addition with reagents, selected using criteria of chemical reactivity, which label at fewer sites than the stains used in previous studies was applied to phase these 50 reflections to produce a one-dimensional electron density distribution map of a single D-repeat of the collagen fibril. This method is not model-dependent and thus constitutes the first unambiguous determination of the meridional phases of type I collagen. © 1989.
Details
- Title
- Phasing the meridional diffraction pattern of type I collagen using isomorphous derivatives
- Authors
- J P Bradshaw (Author) - University of Edinburgh, United KingdomA Miller (Author) - University of Edinburgh, United KingdomTim J Wess (Author) - University of Edinburgh, United Kingdom
- Publication details
- Journal of Molecular Biology, Vol.205(4), pp.685-694
- Publisher
- Academic Press
- Date published
- 1989
- DOI
- 10.1016/0022-2836(89)90314-8
- ISSN
- 0022-2836; 0022-2836
- Organisation Unit
- Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
- Language
- English
- Record Identifier
- 99451393002621
- Output Type
- Journal article
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- Web Of Science research areas
- Biochemistry & Molecular Biology