Peptide products of the atrial gland are not water-borne reproductive pheromones during egg laying in Aplysia

D B G Akalal; Scott F Cummins; S D Painter; G T Nagle

doi:10.1016/j.peptides.2003.06.001

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Peptide products of the atrial gland are not water-borne reproductive pheromones during egg laying in Aplysia

Journal article

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Peptide products of the atrial gland are not water-borne reproductive pheromones during egg laying in Aplysia

D B G Akalal, Scott F Cummins, S D Painter and G T Nagle

Peptides, Vol.24(8), pp.1117-1122

2003

DOI: https://doi.org/10.1016/j.peptides.2003.06.001

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Abstract

ADA

adenosine deaminase

adenosine deaminase-related growth factor

aplysia

atrial gland

egg-laying hormone

MDGF

Mollusk-derived growth factor

Mate attraction in Aplysia involves long-distance water-borne signaling via the secretion of the peptide pheromone attractin from the exocrine albumen gland during egg laying. Previous studies have shown that a second exocrine organ, the atrial gland, produces abundant egg-laying hormone (ELH) precursor-related peptides and mollusk-derived growth factor (MDGF), and crude extracts of the atrial gland are attractive in T-maze attraction assays. However, it is not known whether these peptides and proteins are secreted during egg laying. In this report, seawater eluates of freshly laid egg cordons were concentrated and fractionated by C18 RP-HPLC, and the resulting major peaks were examined by amino acid compositional analysis, microsequence analysis, and electrospray mass spectrometry. Concentrated egg cordon eluates were also examined by immunoblot analysis using anti-MDGF antisera as probe. The combined data demonstrated that the atrial gland of Aplysia californica does not secrete detectable levels of either ELH precursor-related peptides or MDGF during egg laying. Although the atrial gland is the last major exocrine organ to make contact with eggs before they are laid, the gland does not appear to secrete water-borne peptide pheromones during egg laying. © 2003 Elsevier Inc. All rights reserved.

Details

Title: Peptide products of the atrial gland are not water-borne reproductive pheromones during egg laying in Aplysia
Authors: D B G Akalal (Author) - University of Texas Medical Branch, United States
Scott F Cummins (Author) - University of Texas Medical Branch, United States
S D Painter (Author) - University of Texas Medical Branch, United States
G T Nagle (Author) - University of Texas Medical Branch, United States
Publication details: Peptides, Vol.24(8), pp.1117-1122
Publisher: Elsevier Inc.
Date published: 2003
DOI: 10.1016/j.peptides.2003.06.001
ISSN: 0196-9781
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450685802621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology; Endocrinology & Metabolism; Pharmacology & Pharmacy