Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge

Kym M Volp; Sarah A Mathews; Peter Timms; Louise M Hafner

doi:10.1046/j.1440-1711.2001.01005.x

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Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge

Journal article

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Peer reviewed

Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge

Kym M Volp, Sarah A Mathews, Peter Timms and Louise M Hafner

Immunology and Cell Biology, Vol.79(3), pp.245-250

2001

DOI: https://doi.org/10.1046/j.1440-1711.2001.01005.x

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Abstract

Immunology

Anthropology

chlamydia psittaci (GPIC)

partial protection

peptide immunization

vaginal secretion antibody

Immunization of female guinea pigs with a chimeric peptide consisting of variable domain IV (VDIV) and a region known as GP8 from the major outer membrane protein of Chlamydophila caviae, formerly Chlamydia psittaci guinea pig inclusion conjunctivitis strain, was performed to assess whether humoral immune responses could be elicited in the reproductive tracts of immunized animals. The C. caviae strain is able to cause a sexually transmitted infection in the guinea pig that closely parallels C. trachomatis infections in humans. The best anti-VDIV antibody response in vaginal secretions was achieved by intraperitoneal priming with subsequent intravaginal boosting (P < 0.001). Dot-blot analyses of vaginal secretions confirmed that these anti-VDIV antibodies, produced against a linear peptide, were able to recognize and bind to whole conformational C. caviae elementary bodies. Following live intravaginal challenge with C. caviae, a significant reduction in the intensity (P = 0.01) and an apparent reduction in the duration of the infection was evident between the guinea pigs immunized with VDIV-GP8 and non-immunized controls.

Details

Title: Peptide immunization of guinea pigs against Chlamydia psittaci (GPIC agent) infection induces good vaginal secretion antibody response, in vitro neutralization and partial protection against live challenge
Authors: Kym M Volp (Author) - Queensland University of Technology
Sarah A Mathews (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Louise M Hafner (Author) - Queensland University of Technology
Publication details: Immunology and Cell Biology, Vol.79(3), pp.245-250
Publisher: Nature Publishing Group
Date published: 2001
DOI: 10.1046/j.1440-1711.2001.01005.x
ISSN: 0818-9641
Copyright note: Copyright © 2001 Nature Publishing Group. The accepted version is reproduced here in accordance with the publishers copyright policy. The definitive version is available from http://www.nature.com/icb/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449478102621
Output Type: Journal article

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