Partial characterization of the PAF-induced soluble factors which mimic the activity of 'early pregnancy factor'

F M Clarke; C Orozco; A V Perkins; I Cock

doi:10.1530/jrf.0.0880459

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Partial characterization of the PAF-induced soluble factors which mimic the activity of 'early pregnancy factor'

Journal article

Peer reviewed

Partial characterization of the PAF-induced soluble factors which mimic the activity of 'early pregnancy factor'

F M Clarke, C Orozco, A V Perkins and I Cock

Journal of Reproduction & Fertility, Vol.88(2), pp.459-466

1990

DOI: https://doi.org/10.1530/jrf.0.0880459

PMID: 2109065

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Abstract

early pregnancy factor

EPF

platelet activating factor

PAF

rosette inhibition assay

eicosanoids

leukotrienes

Platelet-activating factor (PAF) stimulated mouse spleen cells to release soluble factors (termed S2 factors) which were capable of inducing increased rosette inhibition titres when applied to fresh mouse spleen cells in the rosette inhibition assay. In this ability the S2 factors mimic that of pregnancy serum, an action previously ascribed to 'early pregnancy factor'. The PAF-stimulated production of these S2 factors was not influenced by inhibitors of cyclooxygenase metabolism, but was completely inhibited by the lipoxygenase inhibitors, diethyl carbamazine and nordihydroguaiaretic acid. The S2 factors had a lipid-like character in that they were extractable in organic solvents. The calcium ionophore A23187 also stimulated the production of these factors which may well be products of the lipoxygenase pathway of arachidonic acid metabolism.

Details

Title: Partial characterization of the PAF-induced soluble factors which mimic the activity of 'early pregnancy factor'
Authors: F M Clarke (Author) - Griffith University
C Orozco (Author) - Griffith University
A V Perkins (Author) - Griffith University
I Cock (Author) - Griffith University
Publication details: Journal of Reproduction & Fertility, Vol.88(2), pp.459-466
Publisher: Society for Reproduction and Fertility
DOI: 10.1530/jrf.0.0880459
ISSN: 1471-7899
PMID: 2109065
Organisation Unit: School of Health and Behavioural Sciences - Legacy; School of Health
Language: English
Record Identifier: 99685194602621
Output Type: Journal article

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