Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice

L A Dunn; David J McMillan; M Batzloff; W Zeng; D C J Jackson; J A Upcroft; P Upcroft; C Olive

doi:10.1016/S0264-410X(02)00206-2

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Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice

Journal article

Peer reviewed

Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice

L A Dunn, David J McMillan, M Batzloff, W Zeng, D C J Jackson, J A Upcroft, P Upcroft and C Olive

Vaccine, Vol.20(21-22), pp.2365-3640

2002

DOI: https://doi.org/10.1016/S0264-410X(02)00206-2

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Abstract

Biological Sciences

Agricultural and Veterinary Sciences

Medical and Health Sciences

group A streptococci

multi-epitope

mucosal delivery

Primary vaccine strategies against group A streptococci (GAS) have focused on the M protein-the target of opsonic antibodies important for protective immunity. We have previously reported protection of mice against GAS infection following parenteral delivery of a multi-epitope vaccine construct, referred to as a heteropolymer. This current report has assessed mucosal (intranasal (i.n.) and oral) delivery of the heteropolymer in mice with regard to the induction and specificity of mucosal and systemic antibody responses, and compared this to parenteral delivery. GAS-specific IgA responses were detected in saliva and gut upon i.n. and oral delivery of the heteropolymer co-administered with cholera toxin B subunit, respectively. High titre serum IgG responses were elicited to the heteropolymer following all routes of delivery when administered with adjuvant. Moreover, as with parenteral delivery, serum IgG antibodies were detected to the individual heteropolymer peptides following i.n. but not oral delivery. These data support the potential of the i.n. route in the mucosal delivery of a GAS vaccine.

Details

Title: Parenteral and mucosal delivery of a novel multi-epitope M protein-based group A streptococcal vaccine construct: investigation of immunogenicity in mice
Authors: L A Dunn (Author) - University of Queensland
David J McMillan (Author) - Queensland Institute of Medical Research
M Batzloff (Author) - Queensland Institute of Medical Research
W Zeng (Author) - University of Melbourne
D C J Jackson (Author) - University of Melbourne
J A Upcroft (Author) - Queensland Institute of Medical Research
P Upcroft (Author) - Queensland Institute of Medical Research
C Olive (Author) - Queensland Institute of Medical Research
Publication details: Vaccine, Vol.20(21-22), pp.2365-3640
Publisher: Elsevier Ltd.
Date published: 2002
DOI: 10.1016/S0264-410X(02)00206-2
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450123202621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Immunology; Medicine, Research & Experimental

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